Armbruster-Moraes E, Ioshimoto L M, Leão E, Zugaib M
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Gynecol Oncol. 1994 Aug;54(2):152-8. doi: 10.1006/gyno.1994.1185.
Amniotic fluids from 37 pregnant women with cervical lesions indicative of human papillomavirus (HPV) infection, detected by histology and slot hybridization, were collected at different times during pregnancy and assayed for HPV using the polymerase chain reaction. HPV DNA amplification was obtained in 24 of 37 samples using general primers. Of the 24 positive cases, 13, 5, and 0 were of HPV types 16, 18, and 33, respectively, as determined by using specific anticontamination primers. A correlation between viral DNA amplification and the cervical intraepithelial neoplasia grade was noted. The age of the pregnant women also seemed important for the putative transplacental HPV transmission. At the moment we are not able to evaluate the significance to the fetus of this early contact with the human papillomaviruses; further investigation is necessary to assess the involvement of these viruses in some embryonal and fetal pathology.
通过组织学和狭缝杂交检测出37名患有提示人乳头瘤病毒（HPV）感染的宫颈病变的孕妇，在孕期不同时间收集羊水，并用聚合酶链反应检测HPV。使用通用引物在37个样本中的24个中获得了HPV DNA扩增。在24例阳性病例中，使用特异性抗污染引物确定，分别有13例、5例和0例为HPV 16型、18型和33型。注意到病毒DNA扩增与宫颈上皮内瘤变分级之间存在相关性。孕妇年龄对于假定的经胎盘HPV传播似乎也很重要。目前我们无法评估这种早期接触人乳头瘤病毒对胎儿的意义；需要进一步研究来评估这些病毒在某些胚胎和胎儿病理学中的作用。
