Ui K, Nishihara S, Sakuma M, Togashi S, Ueda R, Miyata Y, Miyake T
Laboratory of Cell Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
In Vitro Cell Dev Biol Anim. 1994 Apr;30A(4):209-16. doi: 10.1007/BF02632042.
From the central nervous system of Drosophila melanogaster 3rd instar larvae, eight continuous cell lines have been established (named ML-DmBG1 to 8). Using ML-DmBG2, single colony isolation was carried out and six colonial clones were obtained. All reacted to the antibody to horseradish peroxidase, which is a neuronal marker in insects. Acetylcholine, a known neurotransmitter in Drosophila, was detected in three of the colonial clones by high performance liquid chromatography. Therefore, it is concluded that the established colonial clones are neural cells originating in the larval central nervous system. Among them, some variation was observed with respect to morphology, acetylcholine content, and reactivity to anti-HRP. The variation may reflect the heterogeneity of cells composing the central nervous system.
从黑腹果蝇三龄幼虫的中枢神经系统中，已建立了八个连续细胞系（命名为ML-DmBG1至8）。使用ML-DmBG2进行了单菌落分离，并获得了六个菌落克隆。所有克隆均对辣根过氧化物酶抗体有反应，辣根过氧化物酶是昆虫中的一种神经元标志物。通过高效液相色谱法在三个菌落克隆中检测到了果蝇中一种已知的神经递质乙酰胆碱。因此，可以得出结论，所建立的菌落克隆是起源于幼虫中枢神经系统的神经细胞。其中，在形态、乙酰胆碱含量和抗HRP反应性方面观察到了一些差异。这种差异可能反映了构成中枢神经系统的细胞的异质性。
