青霉属菌株产生的新型抗球虫剂海那菌素A、B和C。I. 生产、分离以及理化和生物学特性

Hynapenes A, B and C, new anticoccidial agents produced by Penicillium sp. I. Production, isolation and physico-chemical and biological properties.

作者信息

Tabata N, Tomoda H, Masuma R, Haneda K, Iwai Y, Omura S

机构信息

Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.

出版信息

J Antibiot (Tokyo). 1993 Dec;46(12):1849-53. doi: 10.7164/antibiotics.46.1849.

DOI:10.7164/antibiotics.46.1849

PMID:8294243

Abstract

Penicillium sp. FO-1611, a soil isolate, was found to produce a series of new anticoccidial compounds. Three active compounds, designated hynapenes A, B and C, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography, gel filtration on Sephadex LH-20 and preparative HPLC. Hynapenes inhibited the growth of Eimeria tenella in an in vitro assay using BHK-21 cells as a host. No schizont in the cells was observed at concentrations ranging above 123 microM, 34.7 microM and 34.7 microM for hynapenes A, B and C, respectively.

摘要

青霉菌株FO - 1611是从土壤中分离得到的，它能产生一系列新的抗球虫化合物。通过溶剂萃取、硅胶柱色谱、Sephadex LH - 20凝胶过滤和制备型高效液相色谱法，从该产生菌的发酵液中分离出了三种活性化合物，分别命名为海纳烯A、B和C。在以BHK - 21细胞为宿主的体外试验中，海纳烯能抑制柔嫩艾美耳球虫的生长。当海纳烯A、B和C的浓度分别高于123微摩尔/升、34.7微摩尔/升和34.7微摩尔/升时，细胞中未观察到裂殖体。