Keddie J S, Hübner G, Slocombe S P, Jarvis R P, Cummins I, Edwards E W, Shaw C H, Murphy D J
Department of Brassica and Oilseeds Research, Cambridge Laboratory, John Innes Centre for Plant Science Research, Norwich, UK.
Plant Mol Biol. 1992 Jun;19(3):443-53. doi: 10.1007/BF00023392.
The sequence of an oleosin gene from Brassica napus has been determined. This gene contains a single intron of 437 bp and encodes a polypeptide of 195 amino acids. The oleosin gene product has an estimated molecular mass of 21.5 kDa and consists of a highly hydrophobic central domain flanked by relatively polar N- and C-terminal domains. The central domain is highly conserved between all oleosins sequenced to date and contains a run of periodically spaced leucine residues similar to that of a leucine-zipper motif. The gene has been shown to be expressed specifically in the embryo, maximally between 9 and 11 weeks after flowering, i.e. during the seed desiccation stage. Two transcriptional start sites have been mapped to -70 and -21 of the ATG and a putative ABA-responsive element and three repeated motifs have been identified in the promoter. These short promoter sequences could correspond to regulatory elements responsible for embryo-specific gene expression. Up to six genes exist in the oleosin gene family.
已确定了来自甘蓝型油菜的油质蛋白基因序列。该基因含有一个437 bp的单一内含子，编码一个由195个氨基酸组成的多肽。油质蛋白基因产物的估计分子量为21.5 kDa，由一个高度疏水的中央结构域和相对极性的N端及C端结构域组成。中央结构域在迄今测序的所有油质蛋白中高度保守，并且含有一串周期性间隔的亮氨酸残基，类似于亮氨酸拉链基序。该基因已被证明在胚胎中特异性表达，在开花后9至11周达到最大表达，即在种子脱水阶段。已确定了两个转录起始位点，分别位于ATG的-70和-21处，并且在启动子中鉴定出一个假定的脱落酸应答元件和三个重复基序。这些短的启动子序列可能对应于负责胚胎特异性基因表达的调控元件。油质蛋白基因家族中存在多达六个基因。
