克氏锥虫的选择性裂解与纯化研究。
Studies on the selective lysis and purification of Trypanosoma cruzi.
作者信息
Nogueira N, Bianco C, Cohn Z
出版信息
J Exp Med. 1975 Jul 1;142(1):224-9. doi: 10.1084/jem.142.1.224.
Abstract
The mechanism by which culture forms of Trypanosoma cruzi are lysed by normal mammalian sera was examined. Lysis is restricted to the epimastigote form of the organism and is not dependent on the presence of agglutinins. Lysis is a complement-dependent process, the activity being generated by the alternate pathway. The selective lysis by serum was exploited to purify viable trypomastigotes by means of centrifugation in an albumin column. Essentially pure trypomastigote populations are now being employed in cell culture experiments.
摘要
对克氏锥虫的培养形式被正常哺乳动物血清裂解的机制进行了研究。裂解作用仅限于该生物体的上鞭毛体形式，且不依赖于凝集素的存在。裂解是一个补体依赖性过程，其活性由替代途径产生。利用血清的选择性裂解作用，通过在白蛋白柱中离心来纯化活的锥鞭毛体。基本上纯的锥鞭毛体群体现在正被用于细胞培养实验。
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