毛细管电泳中低能β发射体的柱后放射性核素检测

Postcolumn radionuclide detection of low-energy beta emitters in capillary electrophoresis.

作者信息

Tracht S, Toma V, Sweedler J V

机构信息

Department of Chemistry, University of Illinois, Urbana 61801.

出版信息

Anal Chem. 1994 Jul 15;66(14):2382-9. doi: 10.1021/ac00086a026.

DOI:10.1021/ac00086a026

PMID:8080111

Abstract

A postcolumn radionuclide detection system for capillary electrophoresis (CE) is described. Eluant from an electrophoresis capillary is directed onto a peptide binding membrane that has been previously coated with a solid scintillator. The membrane is moved in a preselected pattern relative to the fixed capillary outlet during electrophoresis. Light emission from scintillation is imaged onto a charge-coupled device (CCD) using a series of 35-mm camera lenses. Detection of two low-energy beta- emitters (35S and 3H) not previously reported for capillary electrophoresis is demonstrated. The separation efficiencies are similar to those obtained with on-line UV detection. The response for 35S-labeled methionine is linear (r2 = 0.996) from 66 amol to 11 fmol. Detection limits are 88 zmol (0.03 Bq) for 32P-labeled analytes, 17 amol (0.94 Bq) for 35S-labeled analytes, and 8 fmol (8.5 Bq) for 3H-labeled analytes.

摘要

本文描述了一种用于毛细管电泳（CE）的柱后放射性核素检测系统。来自电泳毛细管的洗脱液被引导至预先涂有固体闪烁体的肽结合膜上。在电泳过程中，该膜相对于固定的毛细管出口以预选模式移动。使用一系列35毫米相机镜头将闪烁产生的光发射成像到电荷耦合器件（CCD）上。展示了对两种此前未报道用于毛细管电泳的低能β发射体（35S和3H）的检测。分离效率与在线紫外检测所获得的效率相似。35S标记的甲硫氨酸的响应在66 amol至11 fmol范围内呈线性（r2 = 0.996）。对于32P标记的分析物，检测限为88 zmol（0.03 Bq）；对于35S标记的分析物，检测限为17 amol（0.94 Bq）；对于3H标记的分析物，检测限为8 fmol（8.5 Bq）。