Franch A, Cassany S, Castellote C, Castell M
Unit of Physiology, Faculty of Pharmacy, University of Barcelona, Spain.
Immunobiology. 1994 Feb;190(1-2):93-104. doi: 10.1016/S0171-2985(11)80285-8.
The aim of this study was to elucidate, during the time course of adjuvant arthritis, the existence of antibodies directed to IgG (rheumatoid factor-like) and antibodies against type II collagen. In a second study, we also studied the relation between antibody production, arthritic process and mycobacteria administration. We have demonstrated the presence of antibodies to IgG and type II collagen by means of ELISA techniques. This reactivity appeared on day 7 post-induction, decreased later, and increased progressively from day 21 until last day studied (day 56 post-induction). We have also quantified antibodies against a soluble fraction of Mycobacterium butyricum, the inductor of the disease. Anti-mycobacteria antibodies appeared during the first seven days after induction, but from day 14, when systemic inflammation began, their levels suddenly increased. There is a positive correlation between anti-mycobacteria antibody levels and articular swelling. Anti-IgG and anti-collagen antibody production was not directly linked to arthritic process since these antibodies were synthesized when M. butyricum was administered intraperitoneally, which does not induce arthritis. Anti-mycobacteria antibody concentration was higher when arthritis induction by mycobacterial was successful than when it was unsuccessful.
本研究的目的是在佐剂性关节炎病程中，阐明针对IgG的抗体（类风湿因子样）和抗II型胶原抗体的存在情况。在第二项研究中，我们还研究了抗体产生、关节炎进程与分枝杆菌给药之间的关系。我们通过ELISA技术证实了抗IgG和抗II型胶原抗体的存在。这种反应性在诱导后第7天出现，随后下降，并从第21天直到研究的最后一天（诱导后第56天）逐渐增加。我们还对针对疾病诱导物丁酸分枝杆菌可溶部分的抗体进行了定量。抗分枝杆菌抗体在诱导后的前七天出现，但从第14天全身炎症开始时起，其水平突然升高。抗分枝杆菌抗体水平与关节肿胀之间存在正相关。抗IgG和抗胶原抗体的产生与关节炎进程没有直接联系，因为当腹腔注射丁酸分枝杆菌时会合成这些抗体，而该菌不会诱发关节炎。当分枝杆菌诱导关节炎成功时，抗分枝杆菌抗体浓度高于诱导失败时。
