Dewhurst S, Krenitsky D M, Dykes C
Department of Microbiology and Immunology, University of Rochester Medical Center, New York 14642.
J Virol. 1994 Oct;68(10):6799-803. doi: 10.1128/JVI.68.10.6799-6803.1994.
A previously identified human herpesvirus 6B (HHV-6B) origin of DNA replication contains two binding sites for the origin-binding protein (OBPH6B). We have investigated the functional significance of these sites by determining the replication efficiencies of mutated origin sequences, using a transient replication assay. The results indicate that both sites are required for DNA replication. In addition, we have tested the functional consequences of linear sequence amplifications in the origin. The data show that tandemized origin elements are more efficiently replicated than single-copy origins. Finally, we have determined the extent of interstrain origin sequence variation that exists among HHV-6 isolates by cloning, sequencing, and analyzing origins from a number of virus isolates, including examples of both HHV-6A and HHV-6B.
先前鉴定出的人类疱疹病毒6B（HHV - 6B）DNA复制起点包含两个与起点结合蛋白（OBPH6B）的结合位点。我们通过使用瞬时复制测定法确定突变起点序列的复制效率，研究了这些位点的功能意义。结果表明，两个位点对于DNA复制都是必需的。此外，我们测试了起点中线性序列扩增的功能后果。数据显示，串联的起点元件比单拷贝起点更有效地复制。最后，我们通过克隆、测序和分析来自多种病毒分离株（包括HHV - 6A和HHV - 6B的实例）的起点，确定了HHV - 6分离株之间存在的株间起点序列变异程度。
