乙型肝炎患者中性粒细胞产生的肿瘤坏死因子α增加。
Increased tumour necrosis factor alpha production by neutrophils in patients with hepatitis B.
作者信息
Fan X, Zhang Z
机构信息
Department of Infectious Diseases, Hsiangya (Yale-China) Hospital, Hunan Medical University, Changsha, Hunan.
出版信息
J Clin Pathol. 1994 Jul;47(7):616-8. doi: 10.1136/jcp.47.7.616.
AIMS
To investigate the role of serum and neutrophil tumour necrosis factor alpha (TNF alpha) in patients with viral hepatitis.
METHODS
The activities of serum and neutrophil TNF alpha were measured using a bioassay of in vitro cytotoxicity against L929 cells in 57 patients with viral hepatitis and 20 healthy blood donors.
RESULTS
Both serum and neutrophil TNF alpha in patients with chronic active hepatitis (CAH) and subacute fulminant hepatitis (SAFH) increased compared with those in normal controls (p < 0.01). No such differences were seen in patients with acute hepatitis. Serum and neutrophil TNF alpha were obviously reduced in patients with CAH and SAFH during convalescence compared with the active period (p < 0.05; p < 0.01). Furthermore, serum TNF alpha was significantly increased in patients with SAFH and complications compared with those without (p < 0.01), and in patients with SAFH who died compared with those who survived (p < 0.01). Neutrophil TNF alpha was significantly higher in patients with SAFH and secondary bacterial infections (p < 0.05).
CONCLUSIONS
Production of serum and neutrophil TNF alpha is increased in patients with CAH and SAFH, suggesting that neutrophil TNF alpha causes liver injury in these patients.
目的
研究血清及中性粒细胞肿瘤坏死因子α（TNFα）在病毒性肝炎患者中的作用。
方法
采用针对L929细胞的体外细胞毒性生物测定法，检测57例病毒性肝炎患者和20名健康献血者血清及中性粒细胞TNFα的活性。
结果
慢性活动性肝炎（CAH）和亚急性重型肝炎（SAFH）患者的血清及中性粒细胞TNFα均高于正常对照组（p<0.01）。急性肝炎患者未见此类差异。与活动期相比，CAH和SAFH患者恢复期的血清及中性粒细胞TNFα明显降低（p<0.05；p<0.01）。此外，与无并发症的SAFH患者相比，有并发症的SAFH患者血清TNFα显著升高（p<0.01），与存活的SAFH患者相比，死亡患者血清TNFα显著升高（p<0.01）。SAFH合并继发细菌感染患者的中性粒细胞TNFα显著更高（p<0.05）。
结论
CAH和SAFH患者血清及中性粒细胞TNFα生成增加，提示中性粒细胞TNFα导致这些患者肝损伤。
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