Miraglia C C, Anderson G, Mintz P D
Department of Pathology, University of Virginia Health Sciences Center, Charlottesville.
Transfusion. 1994 Sep;34(9):775-8. doi: 10.1046/j.1537-2995.1994.34994378278.x.
Transfusion-associated graft-versus-host disease can be prevented by gamma radiation of blood components. The increased use of blood components donated for patients by their family members has resulted in an increased demand for the storage and handling of irradiated units, and the ability to freeze the cells would allow storage beyond their current expiration date.
To assess the effect of freezing and deglycerolization on irradiated red cells, studies of autologous radiolabeled red cell recovery were performed using normal volunteers. Each unit of CPDA-1 red cells was immediately divided into two equal volumes. Further handling of each half was identical except that one was irradiated (3500 cGy). The units were grouped under three protocols: I, irradiated on Day 0 and frozen on Day 5 (n = 4); II, irradiated on Day 7, rejuvenated, and frozen on Day 14 (n = 5); and III, irradiated on Day 14, rejuvenated, and frozen on Day 18 (n = 3). All cells were frozen for 3 to 10 months at -80 degrees C.
Irradiated and control units showed no significant differences in supernatant potassium or hemoglobin. Autologous 24-hour posttransfusion recoveries (mean +/- SD) for the three groups were: I, 89.7 +/- 5.6 percent (control, 90.6 +/- 3.2%); II, 85.3 +/- 5.7 percent (control, 83.7 +/- 3.0%); and III, 79.5 +/- 1.4 percent (control, 82.6 +/- 5.2%).
Irradiated red cells can be frozen after being stored under various conditions and can still meet established guidelines requiring 75-percent recovery 24 hours after transfusion.
通过对血液成分进行γ射线照射可预防输血相关移植物抗宿主病。由患者家属为其捐献血液成分的使用增加，导致对辐照单位储存和处理的需求增加，而细胞冷冻能力将使储存期超过当前有效期。
为评估冷冻和去甘油化对辐照红细胞的影响，使用正常志愿者进行了自体放射性标记红细胞回收研究。每单位CPDA - 1红细胞立即分成两个等体积部分。除了其中一部分进行照射（3500 cGy）外，两半的进一步处理相同。这些单位按照三个方案分组：方案I，第0天照射并于第5天冷冻（n = 4）；方案II，第7天照射、复壮并于第14天冷冻（n = 5）；方案III，第14天照射、复壮并于第18天冷冻（n = 3）。所有细胞在-80℃下冷冻3至10个月。
辐照组和对照组在上清液钾或血红蛋白方面无显著差异。三组的自体输血后24小时回收率（均值±标准差）分别为：方案I，89.7±5.6%（对照组为90.6±3.2%）；方案II，85.3±5.7%（对照组为83.7±3.0%）；方案III，79.5±1.4%（对照组为82.6±5.2%）。
辐照红细胞在不同条件下储存后可冷冻，并且仍能满足输血后24小时回收率达到75%的既定标准。
