Quinn A G, McLelland J, Essex T, Farr P M
University Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
Acta Derm Venereol. 1993 Feb;73(1):21-5. doi: 10.2340/00015555732125.
Responses to a range of doses of common contact dermatitis-producing allergens were measured using a novel scanning laser Doppler velocimeter and three commonly used conventional measurement techniques. The techniques were compared in terms of sensitivity, measurement error, range of the linear portion of the dose-response curve and ease of use. The detection thresholds of the objective methods did not differ significantly and did not detect responses at concentrations less than those required to produce a visible response. Of the objective methods the range of linearity was greatest when reactions were measured using change in skin fold thickness, erythema or area of inflammation. Measurement error was greatest with measurements made using the conventional laser Doppler velocimeter. Present instrumental methods are no more sensitive than visual assessment in the reading of patch test reactions. The conventional laser Doppler velocimeter was least suited for measurement of allergic contact hypersensitivity reactions as readings are time-consuming, show detectable changes over a more limited range of allergen concentration, and have a larger measurement error than the other methods. There is no single best method for measuring allergic contact hypersensitivity reactions. Useful data over a wide range of allergen concentrations can best be obtained by measurement of skin fold thickness, erythema or area of reaction using the scanning laser Doppler velocimeter. The scanning laser Doppler velocimeter has the added advantages of being able to measure area of reaction without contact with the skin surface and to measure reactions at all skin sites.
使用一种新型扫描激光多普勒测速仪和三种常用的传统测量技术，测量了对一系列常见引起接触性皮炎的变应原剂量的反应。对这些技术在灵敏度、测量误差、剂量反应曲线线性部分的范围以及易用性方面进行了比较。客观方法的检测阈值没有显著差异，并且在浓度低于产生可见反应所需浓度时无法检测到反应。在客观方法中，当使用皮肤褶皱厚度变化、红斑或炎症面积来测量反应时，线性范围最大。使用传统激光多普勒测速仪进行测量时，测量误差最大。目前的仪器方法在读取斑贴试验反应方面并不比视觉评估更敏感。传统激光多普勒测速仪最不适合测量过敏性接触超敏反应，因为读数耗时，在变应原浓度范围更有限时显示可检测到的变化，并且比其他方法有更大的测量误差。没有单一的最佳方法来测量过敏性接触超敏反应。通过使用扫描激光多普勒测速仪测量皮肤褶皱厚度、红斑或反应面积，可以在广泛的变应原浓度范围内获得有用的数据。扫描激光多普勒测速仪还具有能够在不接触皮肤表面的情况下测量反应面积以及测量所有皮肤部位反应的额外优势。
