Gawkrodger D J, McDonagh A J, Wright A L
Department of Dermatology, University of Sheffield, Royal Hallamshire Hospital, UK.
Contact Dermatitis. 1991 Mar;24(3):172-7. doi: 10.1111/j.1600-0536.1991.tb01693.x.
The laser-Doppler blood flow and erythema index were assessed in 16 patch test reactions caused by irritants (1% aq. sodium lauryl sulphate and 1% aq. benzalkonium chloride) and in 13 varied allergic reactions, at 2 or 4 days. Both irritant and allergic responses produced statistically significant increases in laser-Doppler flow index and erythema index compared to control sites (p less than 0.05 or less, using the Wilcoxon rank-sum test). A disproportionately greater increase in erythema index than in laser-Doppler flow was seen in mild irritant reactions, compared to allergic, though the two could not be reliably distinguished by these tests. 2 non-allergic nickel sulphate sites produced an increase in both blood flow and erythema without any clinical change. Petrolatum alone produced no significant change in either measurement. The laser-Doppler flow showed an overall correlation with the erythema index (product moment method; r = 0.55, p less than 0.001), but there was little correlation between these indices and patch test reactivity as judged clinically by conventional scoring. Generally, allergic and irritant patch test reactions could not be differentiated on the basis of laser-Doppler flow or erythema index. However, the disproportionate increase in erythema index over laser-Doppler flow index for mild irritant responses warrants further study in other irritant models.
在2天或4天时，对由刺激物（1%的十二烷基硫酸钠水溶液和1%的苯扎氯铵水溶液）引起的16例斑贴试验反应以及13例不同的过敏反应进行了激光多普勒血流和红斑指数评估。与对照部位相比，刺激反应和过敏反应均使激光多普勒血流指数和红斑指数出现具有统计学意义的增加（使用Wilcoxon秩和检验，p小于或等于0.05）。与过敏反应相比，在轻度刺激反应中观察到红斑指数的增加比激光多普勒血流的增加更为显著，不过通过这些试验无法可靠地区分二者。2个非过敏性硫酸镍部位的血流和红斑均增加，但无任何临床变化。单独使用凡士林在两项测量中均未产生显著变化。激光多普勒血流与红斑指数总体呈相关性（积差相关法；r = 0.55，p小于0.001），但根据传统评分临床判断，这些指数与斑贴试验反应性之间几乎没有相关性。一般而言，无法根据激光多普勒血流或红斑指数区分过敏和刺激斑贴试验反应。然而，轻度刺激反应中红斑指数相对于激光多普勒血流指数的不成比例增加值得在其他刺激模型中进一步研究。
