酿酒酵母法尼基二磷酸合酶保守序列中赖氨酸到谷氨酸突变的表征
Characterization of a lysine-to-glutamic acid mutation in a conservative sequence of farnesyl diphosphate synthase from Saccharomyces cerevisiae.
作者信息
Blanchard L, Karst F
机构信息
Laboratoire de Biochimie et Génétique des Microorganismes, Université de Poitiers, France.
出版信息
Gene. 1993 Mar 30;125(2):185-9. doi: 10.1016/0378-1119(93)90326-x.
The mutant gene erg20-2 was isolated from a yeast strain defective in farnesyl diphosphate synthase (FPPS). This strain had the unusual property of excreting prenyl alcohols such as geraniol. The nucleotide (nt) sequence, compared with that of the wild-type gene, showed a single nt change, resulting in a Lys197-->Glu substitution in FPPS which is directly involved in terpenic alcohol formation. In addition, disruption of ERG20 revealed that in yeast no other prenyl transferase is able to synthesize the FPP molecules required for essential non-sterol metabolites.
突变基因erg20 - 2是从一株法尼基二磷酸合酶（FPPS）缺陷的酵母菌株中分离出来的。该菌株具有排泄香叶醇等异戊烯醇的特殊性质。与野生型基因的核苷酸（nt）序列相比，发现有一个单核苷酸变化，导致FPPS中第197位赖氨酸（Lys197）被谷氨酸（Glu）取代，这直接参与了萜醇的形成。此外，ERG20基因的破坏表明，在酵母中没有其他异戊烯基转移酶能够合成必需的非甾醇代谢物所需的FPP分子。
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