编码核糖体DNA的枯草芽孢杆菌基因杂交模式的差异取决于DNA制备方法。
Differences in the hybridization pattern of Bacillus subtilis genes coding for rDNA depend on the method of DNA preparation.
作者信息
Waterhouse R N, Glover L A
机构信息
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, Scotland.
出版信息
Appl Environ Microbiol. 1993 Mar;59(3):919-21. doi: 10.1128/aem.59.3.919-921.1993.
Abstract
Three different methods of DNA isolation (organic deproteinization, potassium acetate deproteinization, and the use of cetyltrimethylammonium bromide) have been used to prepare DNA from Bacillus subtilis. Subsequent hybridization with an rDNA probe (DNA coding for rRNA) produces different patterns, which mirror those previously reported to indicate an rDNA deletion.
摘要
已使用三种不同的DNA分离方法（有机脱蛋白法、醋酸钾脱蛋白法和使用十六烷基三甲基溴化铵）从枯草芽孢杆菌中制备DNA。随后与rDNA探针（编码rRNA的DNA）杂交产生不同的模式，这些模式与先前报道的表明rDNA缺失的模式一致。
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