Bourre J M, Dumont O, Pascal G, Durand G
Institut National de la Santé et de la Recherche Médicale, INSERM unité 26, Hôpital Fernand Widal, Paris, France.
J Nutr. 1993 Jul;123(7):1313-9. doi: 10.1093/jn/123.7.1313.
We have previously determined the dietary alpha-linolenic requirement for membrane synthesis in the developing animal. This study measures the dietary requirement for maintaining normal membrane composition in adult rats, as determined by 22:6(n-3) (docosahexaenoic acid) concentration. Sixty-day-old rats, previously fed a diet containing both linoleic and alpha-linolenic acid, were divided into nine groups, each receiving different quantities of alpha-linolenic acid but the same amount of linoleic acid. They were killed 4 wk after initiation of the new diet to determine the minimum quantity of alpha-linolenic acid required in the diet for maintaining the 22:6(n-3) tissue concentration in brain (whole tissue, myelin and nerve endings), liver and heart. The minimal amount of dietary alpha-linolenic acid that maintained the maximal 22:6(n-3) level and minimal 22:5(n-6) level in tissues was considered to be the dietary requirement. The quantity was found to be 1.30 g/kg diet (0.26% of dietary energy). It was lower than that found for the developing animal (0.4% of energy). At lower quantities of dietary alpha-linolenic acid, 22:6(n-3) was replaced by 22:5(n-6) in the organs examined, except in nervous tissue, in which 22:6(n-3) was highly preserved.
我们之前已经确定了发育中动物膜合成所需的膳食α-亚麻酸。本研究测量了成年大鼠维持正常膜组成所需的膳食需求量，该需求量通过22:6(n-3)（二十二碳六烯酸）浓度来确定。将先前喂食含有亚油酸和α-亚麻酸饮食的60日龄大鼠分为9组，每组接受不同量的α-亚麻酸，但亚油酸量相同。在开始新饮食4周后将它们处死以确定饮食中维持大脑（全组织、髓磷脂和神经末梢）、肝脏和心脏中22:6(n-3)组织浓度所需的α-亚麻酸的最小量。维持组织中最大22:6(n-3)水平和最小22:5(n-6)水平的膳食α-亚麻酸的最小量被认为是膳食需求量。发现该量为1.30 g/kg饮食（占膳食能量的0.26%）。它低于发育中动物的需求量（能量的0.4%）。在膳食α-亚麻酸量较低时，在所检查的器官中，22:6(n-3)被22:5(n-6)取代，但神经组织除外，其中22:6(n-3)高度保留。
