Bignami G S
Hawaii Biotechnology Group, Inc., Aiea 96701.
Toxicon. 1993 Jun;31(6):817-20. doi: 10.1016/0041-0101(93)90389-z.
The hemolytic action of palytoxin was exploited to develop a simple, sensitive assay with specificity based on a palytoxin neutralizing monoclonal antibody. Suspensions of murine erythrocytes incubated at 37 degrees C in round-bottom microtiter trays formed visible cell pellets which could be lysed by palytoxin. Hemolysis by palytoxin was time- and temperature-dependent, with a 24 hr detection limit of 1 pg/ml. The assay selectively detected palytoxin in a crude extract of Palythoa tuberculosa.
