Denamur E, Picard B, Decoux G, Denis J B, Elion J
Laboratoire de Biochimie Génétique, Hôpital Robert Debré, Paris, France.
FEMS Microbiol Lett. 1993 Jul 1;110(3):275-80. doi: 10.1111/j.1574-6968.1993.tb06334.x.
Two hundred and fifty seven human clinical Pseudomonas aeruginosa strains isolated between 1984 and 1990 in several regions of France, as well as two reference strains, were studied by computer-assisted statistical analysis of the data from their esterase electrophoretic patterns and rrn restriction fragment length polymorphisms. No correlation was found between the two sets of data except for some strains of serotype O12 which, thus, may constitute a distinct group within the species. This absence of correlation indicates a high gene flow rate within human isolates of the P. aeruginosa species. A possible explanation is that, because of an as yet unidentified selective advantage, the esterase loci are a major target for recombinational events. Alternatively, horizontal genetic transfers between strains may have occurred at so high a rate that the clonal structure usually observed in bacterial populations has been disrupted. This study highlights clearly the need for caution in inferring bacterial population structure from any single class of genetic markers.
对1984年至1990年间在法国多个地区分离出的257株人类临床铜绿假单胞菌菌株以及两株参考菌株，通过对其酯酶电泳图谱和rrn限制性片段长度多态性数据进行计算机辅助统计分析。除了一些O12血清型菌株外，两组数据之间未发现相关性，因此，这些O12血清型菌株可能构成该物种内的一个独特群体。这种缺乏相关性表明铜绿假单胞菌物种的人类分离株内基因流动率很高。一种可能的解释是，由于尚未确定的选择优势，酯酶基因座是重组事件的主要靶点。或者，菌株之间的水平基因转移可能以如此高的速率发生，以至于通常在细菌群体中观察到的克隆结构被破坏。这项研究清楚地强调了在从任何单一类别的遗传标记推断细菌群体结构时需要谨慎。
