Institut de Génétique et Microbiologie, University Paris-Sud, UMR 8621, 91405, Orsay, France.
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2247-56. doi: 10.1007/s10096-012-1562-5. Epub 2012 Feb 11.
Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) has been shown to provide a high level of information for epidemiological investigations and the follow-up of Pseudomonas aeruginosa chronic infection. In the present study, an automatized MLVA assay has been developed for the analysis of 16 VNTRs in two multiplex polymerase chain reactions (PCRs), followed by capillary electrophoresis. The result in the form of a code is directly usable for clustering analyses. This MLVA-16(Orsay) scheme was applied to the genotyping of 83 isolates from eight cystic fibrosis patients, demonstrating that the same genotype persisted during eight years of chronic infection in the majority of cases. Comparison with pulsed-field gel electrophoresis (PFGE) analysis showed that both methods were congruent, MLVA providing, in some cases, additional informativity. The evolution of strains during long-term infection was revealed by the presence of VNTR variants.
多位点可变数目串联重复序列（VNTR）分析（MLVA）已被证明为流行病学调查和铜绿假单胞菌慢性感染的后续研究提供了高度信息。在本研究中，开发了一种自动 MLVA 分析方法，用于在两个多重聚合酶链反应（PCR）中分析 16 个 VNTR，然后进行毛细管电泳。以代码形式的结果可直接用于聚类分析。该 MLVA-16（Orsay）方案应用于 8 例囊性纤维化患者的 83 株分离株的基因分型，结果表明在大多数情况下，在 8 年的慢性感染期间，相同的基因型持续存在。与脉冲场凝胶电泳（PFGE）分析的比较表明，两种方法均一致，在某些情况下，MLVA 提供了更多的信息。VNTR 变异的存在揭示了长期感染过程中菌株的演变。
