Montero A, Bragado J, Alonso R M, García L J, Calvo J J, López M A
Departamento de Fisiología y Farmacología, Universidad de Salamanca, Spain.
Rev Esp Fisiol. 1993 Jun;49(2):93-9.
The effect of rapid wash-out of the duodenum with phosphate buffered saline on exocrine pancreatic secretion and plasma levels of secretin, VIP, gastrin and CCK was studied. Furthermore, the possible nervous role in this effect was checked after atropine and hexamethonium treatment. Rapid wash-out significantly increased protein output (35.0 micrograms/min, in the control group without duodenal perfusion and 72.15 micrograms/min, in the perfused group) and the plasma levels of CCK (from 5.2 to 13.17 fmol/ml). Intravenous infusion of atropine significantly reduced the protein output (from 78.19 to 32.45 micrograms/min) and the plasma levels of CCK (from 10.1 to 5.55 fmol/ml), with no change in the remaining parameters in the intraduodenally perfused group. Intravenous administration of hexamethonium significantly stimulated hydroelectrolyte secretion (from 6.99 to 15.15 microliters/min) and the plasma levels of VIP (from 4.8 to 7.3 fmol/ml) and reduced the protein output (from 61.47 to 30.75 micrograms/min) and the plasma levels of CCK (from 14.56 to 6.25 fmol/ml) in the intraduodenally perfused group. Our results suggest that, in the interdigestive state, the exocrine pancreatic secretion of the rabbit is tonically inhibited. This inhibition can be divided into two different mechanisms: on the one hand there is a decrease in enzyme secretion produced by a duodenal factor and mediated by CCK and muscarinic mechanisms and on the other, there is an inhibition of hydroelectrolyte secretion with no duodenal participation which is probably controlled by nervous non-muscarinic mechanisms and VIP involvement.
研究了用磷酸盐缓冲盐水快速冲洗十二指肠对外分泌性胰腺分泌以及血浆中促胰液素、血管活性肠肽、胃泌素和胆囊收缩素水平的影响。此外，在给予阿托品和六甲铵治疗后，检查了这种作用中可能的神经作用。快速冲洗显著增加了蛋白质输出量（未进行十二指肠灌注的对照组为35.0微克/分钟，灌注组为72.15微克/分钟）以及血浆中胆囊收缩素的水平（从5.2皮摩尔/毫升升至13.17皮摩尔/毫升）。静脉注射阿托品显著降低了蛋白质输出量（从78.19微克/分钟降至32.45微克/分钟）以及血浆中胆囊收缩素的水平（从10.1皮摩尔/毫升降至5.55皮摩尔/毫升），而十二指肠内灌注组的其余参数没有变化。静脉注射六甲铵显著刺激了水电解质分泌（从6.99微升/分钟升至15.15微升/分钟）以及血浆中血管活性肠肽的水平（从4.8皮摩尔/毫升升至7.3皮摩尔/毫升），并降低了十二指肠内灌注组的蛋白质输出量（从61.47微克/分钟降至30.75微克/分钟）以及血浆中胆囊收缩素的水平（从14.56皮摩尔/毫升降至6.25皮摩尔/毫升）。我们的结果表明，在消化间期，家兔的外分泌性胰腺分泌处于紧张性抑制状态。这种抑制可分为两种不同机制：一方面，由十二指肠因子产生并由胆囊收缩素和毒蕈碱机制介导的酶分泌减少；另一方面，在没有十二指肠参与的情况下对水电解质分泌的抑制，这可能由非毒蕈碱神经机制和血管活性肠肽参与控制。
