Jarvis P, Lister C, Szabo V, Dean C
AFRC IPSR Cambridge Laboratory, John Innes Centre, Norwich, UK.
Plant Mol Biol. 1994 Feb;24(4):685-7. doi: 10.1007/BF00023565.
Konieczny and Ausubel have described a technique whereby Arabidopsis thaliana loci can be rapidly mapped to one of the ten chromosome arms using a small number of F2 progeny from crosses between the ecotypes Landsberg erecta and Columbia. The technique involves the use of 18 co-dominant, cleaved amplified polymorphic sequence (CAPS) markers which are evenly distributed throughout the Arabidopsis genome. We have mapped these 18 markers using recombinant inbred (RI) lines generated in our laboratory. These data enable a better integration of loci mapped relative to the CAPS markers into the restriction fragment length polymorphism (RFLP) map generated using Arabidopsis RI lines.
科涅茨尼和奥苏贝尔描述了一种技术,利用生态型直立叶兰茨贝格和哥伦比亚之间杂交产生的少量F2后代,可将拟南芥基因座快速定位到十条染色体臂中的一条上。该技术使用了18个共显性的酶切扩增多态性序列(CAPS)标记,这些标记均匀分布于整个拟南芥基因组。我们利用在我们实验室产生的重组自交(RI)系对这18个标记进行了定位。这些数据能够使相对于CAPS标记定位的基因座更好地整合到使用拟南芥RI系产生的限制性片段长度多态性(RFLP)图谱中。