利用重组自交系对拟南芥中一组新的129个限制性片段长度多态性（RFLP）标记进行分离和定位。

Isolation and mapping of a new set of 129 RFLP markers in Arabidopsis thaliana using recombinant inbred lines.

作者信息

Liu Y G, Mitsukawa N, Lister C, Dean C, Whittier R F

机构信息

Mitsui Plant Biotechnology Research Institute, Tsukuba, Japan.

出版信息

Plant J. 1996 Oct;10(4):733-6. doi: 10.1046/j.1365-313x.1996.10040733.x.

DOI:10.1046/j.1365-313x.1996.10040733.x

PMID:8893548

Abstract

A new collection of 129 Arabidopsis thaliana RFLP markers has been established based upon DNA fragments cloned in the pUC119 plasmid vector and insert end sequences of P1 clones. Dominant/null alleles affecting low-copy number sequences account for nine of the mapped polymorphisms, suggesting that deletions are not rare in A. thaliana. Recombinant inbred (RI) lines were used for mapping these marker loci. RI line-based mapping allows integration of this set of markers with markers previously reported as well as with any markers mapped in the future using this replenishable mapping resource. These markers are useful for map-based gene isolation and genome physical mapping in A. thaliana as well as studies of chromosome colinearity (synteny) with related species.

摘要

基于克隆于pUC119质粒载体的DNA片段以及P1克隆的插入末端序列，建立了一个包含129个拟南芥RFLP标记的新集合。影响低拷贝数序列的显性/无效等位基因占已定位多态性的9个，这表明缺失在拟南芥中并不罕见。重组自交（RI）系被用于这些标记位点的定位。基于RI系的定位使得这组标记能够与先前报道的标记以及未来使用这种可补充的定位资源定位的任何标记整合。这些标记对于拟南芥中基于图谱的基因分离和基因组物理图谱绘制以及与相关物种的染色体共线性（同线性）研究很有用。