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Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.

作者信息

Svenningsen B A

出版信息

Mol Gen Genet. 1975;137(4):289-304. doi: 10.1007/BF00703255.

DOI:10.1007/BF00703255
PMID:810659
Abstract

The four enzymes deoxyriboaldolase, thymidine phosporylase, deoxyribomutase, and purine nucleoside phosphorylase have been synthesized in substantial amounts in a DNA-dependent in vitro system programmed with DNA containing the deo operon. The synthesis is greatly stimulated by deoxyribose-5-phosphate and cyclic AMP indicating that the deoR repressor and the catabolite activating protein (CAP) are highly active under our cell-free conditions. In contrast it has not yet been possible to observe a reproducible effect of the cytR repressor in vitro. The sequential appearance of active enzymes has confirmed the direction of transcription as being dra-tpp-drm-pup and has indicated that the four genes are transcribed into a single tetracistronic message.

摘要

相似文献

1
Regulated in vitro synthesis of the enzymes of the deo operon of Escerichia coli. properties of the DNA directed system.
Mol Gen Genet. 1975;137(4):289-304. doi: 10.1007/BF00703255.
2
Regulation of the deo operon in Escherichia coli: the double negative control of the deo operon by the cytR and deoR repressors in a DNA directed in vitro system.大肠杆菌中deo操纵子的调控：在体外DNA定向系统中，cytR和deoR阻遏物对deo操纵子的双重负调控。
Mol Gen Genet. 1978 Feb 16;159(2):191-202. doi: 10.1007/BF00270893.
3
Multiple regulation of nucleoside catabolizing enzymes: effects of a polar dra mutation on the deo enzymes.核苷分解代谢酶的多重调控：极性dra突变对脱氧酶的影响
Mol Gen Genet. 1976 Jul 23;146(2):139-45. doi: 10.1007/BF00268082.
4
Evidence for the existence of three promoters for the deo operon of Escherichia coli K12 in vitro.体外存在大肠杆菌K12的deo操纵子的三个启动子的证据。
J Mol Biol. 1979 Sep 5;133(1):1-17. doi: 10.1016/0022-2836(79)90248-1.
5
Multiple regulation of nucleoside catabolizing enzymes: regulation of the deo operon by the cytR and deoR gene products.
Mol Gen Genet. 1975;137(4):327-35. doi: 10.1007/BF00703258.
6
[Disturbance of the tandem promotor functioning of the Escherichia coli K-12 deo-operon in the genome of the rho15(ts) mutant for the transcription termination factor].[rho15（ts）转录终止因子突变体基因组中大肠杆菌K-12脱氧操纵子串联启动子功能的紊乱]
Genetika. 1981;17(10):1719-29.
7
[Mutation affecting the promotor region of the Escherichia coli K-12 deo-operon].[影响大肠杆菌K-12脱氧操纵子启动子区域的突变]
Genetika. 1981;17(5):782-93.
8
[Regulation of the activity of Escherichia coli deo-operon structural genes: the mutation mapped within the operon boundaries and affecting drm and pup gene activity].[大肠杆菌脱氧操纵子结构基因活性的调控：定位在操纵子边界内且影响drm和pup基因活性的突变]
Genetika. 1978;14(12):2091-101.
9
Influence of the rho-15 temperature-sensitive (ts) mutation on the expression of the deo-operon in Escherichia coli.rho-15温度敏感（ts）突变对大肠杆菌中脱氧操纵子表达的影响。
Mol Gen Genet. 1982;187(1):157-61. doi: 10.1007/BF00384399.
10
Genetic analysis of thymidine-resistant and low-thymine-requiring mutants of Escherichia coli K-12 induced by bacteriophage Mu-1.由噬菌体Mu-1诱导的大肠杆菌K-12抗胸腺嘧啶和低胸腺嘧啶需求突变体的遗传分析。
J Bacteriol. 1975 Feb;121(2):475-84. doi: 10.1128/jb.121.2.475-484.1975.

引用本文的文献

1
Influence of the rho-15 temperature-sensitive (ts) mutation on the expression of the deo-operon in Escherichia coli.rho-15温度敏感（ts）突变对大肠杆菌中脱氧操纵子表达的影响。
Mol Gen Genet. 1982;187(1):157-61. doi: 10.1007/BF00384399.
2
The internal regulated promoter of the deo operon of Escherichia coli K-12.大肠杆菌K-12中deo操纵子的内部调控启动子。
Nucleic Acids Res. 1984 Jul 11;12(13):5211-24. doi: 10.1093/nar/12.13.5211.
3
The primary structure of the DeoR repressor from Escherichia coli K-12.来自大肠杆菌K-12的DeoR阻遏物的一级结构。

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
3
On the catabolism of deoxyribonucleosides in cells and cell extracts of Escherichia coli.关于大肠杆菌细胞及细胞提取物中脱氧核糖核苷的分解代谢
Nucleic Acids Res. 1985 Aug 26;13(16):5927-36. doi: 10.1093/nar/13.16.5927.
4
Multiple regulation of nucleoside catabolizing enzymes: effects of a polar dra mutation on the deo enzymes.核苷分解代谢酶的多重调控：极性dra突变对脱氧酶的影响
Mol Gen Genet. 1976 Jul 23;146(2):139-45. doi: 10.1007/BF00268082.
5
Recalibrated linkage map of Escherichia coli K-12.大肠杆菌K-12的重新校准连锁图谱。
Bacteriol Rev. 1976 Mar;40(1):116-67. doi: 10.1128/br.40.1.116-167.1976.
6
Thymidine uptake and utilization in Escherichia coli: a new gene controlling nucleoside transport.大肠杆菌中胸苷的摄取与利用：一个控制核苷转运的新基因
J Bacteriol. 1976 May;126(2):814-22. doi: 10.1128/jb.126.2.814-822.1976.
7
Pyrimidine nucleoside analogues as inducers of pyrimidine nucleoside catabolizing enzymes in Salmonella typhimurium.嘧啶核苷类似物作为鼠伤寒沙门氏菌中嘧啶核苷分解代谢酶的诱导剂。
Mol Biol Rep. 1975 Dec;2(4):295-301. doi: 10.1007/BF00357016.
8
Insertion of bacteriophage lambda into the deo operon of Escherichia coli K-12 and isolation of plaque-forming lambdadeo+ transducing bacteriophages.将噬菌体λ插入大肠杆菌K-12的deo操纵子并分离形成噬菌斑的λdeo⁺转导噬菌体。
J Bacteriol. 1978 Nov;136(2):668-81. doi: 10.1128/jb.136.2.668-681.1978.
9
Reevaluation of the mode of action of streptolydigin in Escherichia coli: induction of transcription termination in vivo.对链霉溶菌素在大肠杆菌中作用模式的重新评估：体内转录终止的诱导
Antimicrob Agents Chemother. 1978 Feb;13(2):234-43. doi: 10.1128/AAC.13.2.234.
10
Regulation of the deo operon in Escherichia coli: the double negative control of the deo operon by the cytR and deoR repressors in a DNA directed in vitro system.大肠杆菌中deo操纵子的调控：在体外DNA定向系统中，cytR和deoR阻遏物对deo操纵子的双重负调控。
Mol Gen Genet. 1978 Feb 16;159(2):191-202. doi: 10.1007/BF00270893.
Eur J Biochem. 1968 Nov;6(3):432-42. doi: 10.1111/j.1432-1033.1968.tb00465.x.
4
Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridine.来自大肠杆菌的胞苷脱氨酶。纯化、性质以及被潜在过渡态类似物3,4,5,6-四氢尿苷的抑制作用。
J Biol Chem. 1971 Dec 25;246(24):7561-5.
5
A regulatory mutant affecting the synthesis of enzymes involved in the catabolism of nucleosides in Escherichia coli.
Mol Gen Genet. 1971;111(1):77-83. doi: 10.1007/BF00286556.
6
Induction of enzymes involed in the catabolism of deoxyribonucleosides and ribonucleosides in Escherichia coli K 12.
Eur J Biochem. 1971 Apr 30;19(4):533-8. doi: 10.1111/j.1432-1033.1971.tb01345.x.
7
A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxynucleosides in Escherichia coli.一张关于大肠杆菌中参与核苷和脱氧核苷分解代谢的四种指定酶的基因图谱。
Mol Gen Genet. 1969 Aug 15;104(4):351-9. doi: 10.1007/BF00334234.
8
Characteristics of the deo operon: role in thymine utilization and sensitivity to deoxyribonucleosides.deo操纵子的特性：在胸腺嘧啶利用中的作用及对脱氧核苷的敏感性
J Bacteriol. 1968 Aug;96(2):501-14. doi: 10.1128/jb.96.2.501-514.1968.
9
The absence of deoxyriboaldolase activity in a thymineless mutant of Escherichia coli strain 15: a possible explanation for the low thymine requirement of some thymineless strains.大肠杆菌15株的一个无胸腺嘧啶突变体中缺乏脱氧核糖醛缩酶活性：对某些无胸腺嘧啶菌株胸腺嘧啶需求低的一种可能解释。
Biochim Biophys Acta. 1967 Mar 29;138(1):217-20. doi: 10.1016/0005-2787(67)90610-7.
10
In vitro synthesis of protein in microbial systems.微生物系统中蛋白质的体外合成。
Annu Rev Genet. 1973;7:267-87. doi: 10.1146/annurev.ge.07.120173.001411.