Dumitrescu O, Kalish M L, Kliks S C, Bandea C I, Levy J A
School of Medicine, University of California, San Francisco 94143-0128.
J Infect Dis. 1994 Feb;169(2):281-8. doi: 10.1093/infdis/169.2.281.
Human immunodeficiency virus type 1 (HIV-1) isolates recovered from infected children in Romania were characterized for their biologic, serologic, and molecular properties. The isolates were from subjects in different clinical states, and all showed cytopathic properties in peripheral blood mononuclear cells and varying kinetics of replication. The isolates grew to varying titers in macrophages and established T cell lines. Serologic evaluation with Romanian sera indicated stronger antibody response to the gp120 of Romanian isolates than to the envelope protein of HIV-1 isolates from other countries. Although there was cross-neutralization among the Romanian isolates, no substantial activity was noted against HIV-1 prototype strains from the United States, Africa, and Thailand. Genetic analysis of the envelope C2-V3 region strongly suggests that the Romanian isolates are a subtype distinct from those assigned to other HIV-1 strains analyzed to date. This finding raises questions about the origin of HIV-1 in Romania.
对从罗马尼亚感染儿童中分离出的1型人类免疫缺陷病毒（HIV-1）毒株进行了生物学、血清学和分子特性鉴定。这些毒株来自处于不同临床状态的受试者，并且在人外周血单核细胞中均表现出细胞病变特性以及不同的复制动力学。这些毒株在巨噬细胞和建立的T细胞系中生长至不同滴度。用罗马尼亚血清进行的血清学评估表明，罗马尼亚毒株的gp120比来自其他国家的HIV-1毒株包膜蛋白引发更强的抗体反应。虽然罗马尼亚毒株之间存在交叉中和作用，但对来自美国、非洲和泰国的HIV-1原型毒株未观察到显著活性。包膜C2-V3区域的基因分析强烈表明，罗马尼亚毒株是一种与迄今分析的其他HIV-1毒株不同的亚型。这一发现引发了关于罗马尼亚HIV-1起源的问题。
