Revol J F, Marchessault R H
Paprican Department, McGill University, Quebec, Canada.
Int J Biol Macromol. 1993 Dec;15(6):329-35. doi: 10.1016/0141-8130(93)90049-r.
Microfibrillar fragments of purified crab and shrimp chitin were prepared by hydrolysis in 3 M HCl at its boiling point (104 degrees C). After removal of the acid by centrifugal washing and dialysis, an ultrasound treatment converts the residual product to a colloidal suspension stabilized by NH3+ charges. When dewatered to a critical concentration, spontaneous formation of a two-phase equilibrium system system occurs. The upper phase (lower concentration) is isotropic and the lower phase is anisotropic. The latter displays chiral nematic order and dries to a solid film which mimics the helicoid organization characteristic of the chitin microfibrils in the cuticle of arthropods.
通过在沸点（104摄氏度）的3 M盐酸中水解制备纯化的蟹和虾几丁质的微纤维片段。通过离心洗涤和透析除去酸后，超声处理将残留产物转化为由NH3+电荷稳定的胶体悬浮液。当脱水至临界浓度时，会自发形成两相平衡体系。上相（较低浓度）是各向同性的，而下相是各向异性的。后者呈现手性向列相序，并干燥成固体薄膜，该薄膜模仿节肢动物表皮中几丁质微纤维的螺旋状组织特征。
