Meyle J, Gültig K, Nisch W
Clinic of Oral Surgery and Periodontology, School of Dentistry and Maxillofacial Surgery, University of Tuebingen, Germany.
J Biomed Mater Res. 1995 Jan;29(1):81-8. doi: 10.1002/jbm.820290112.
Contact guidance induced by the surface topography of the underlying substratum influences the interaction of tissue cells with implanted material. It was the aim of this study to compare the reaction of different human cells on the same surface microtexture. After staining with fluoresceinediacetate, the orientation of human fibroblasts, gingival keratinocytes, neutrophils, monocytes, and macrophages on a regular surface microstructure of 1 microns pitch and 1 microns depth was analyzed by fluorescence microscopy. Contact guidance could not be observed in the experiments with keratinocytes and neutrophils, but 100% of the fibroblasts and approximately 20% of the monocytes and macrophages reacted with alignment. After 2 h some of the macrophages extended long dendritic cellular processes parallel to the long axis of the microstructures.
由下层基质的表面形貌诱导的接触导向影响组织细胞与植入材料的相互作用。本研究的目的是比较不同人类细胞在相同表面微观结构上的反应。用荧光素二乙酸酯染色后，通过荧光显微镜分析人成纤维细胞、牙龈角质形成细胞、中性粒细胞、单核细胞和巨噬细胞在间距为1微米、深度为1微米的规则表面微观结构上的取向。在角质形成细胞和中性粒细胞的实验中未观察到接触导向，但100%的成纤维细胞以及约20%的单核细胞和巨噬细胞发生了排列反应。2小时后，一些巨噬细胞沿着微观结构的长轴延伸出长的树突状细胞突起。
