Meyle J, Wolburg H, von Recum A F
Clinic of Oral Surgery and Periodontics, School of Dentistry and Maxillofacial Surgery, University of Tuebingen, Germany.
J Biomater Appl. 1993 Apr;7(4):362-74. doi: 10.1177/088532829300700404.
Contact guidance induced by the topographical properties of the underlying substratum is of great importance in morphogenesis and also influences the interaction of tissue cells with implanted material. A large body of evidence has accumulated since the first detection of this phenomenon in 1910. Several major hypotheses have been developed to explain the observed cell behaviour. The technological progress enabled researchers to produce pure substrata with a defined and controlled surface microgeometry. Based on these specimens, it could be demonstrated that cytoskeletal structures and receptors forming focal adhesions most likely are involved in contact guidance. In a study using human gingival fibroblasts, the reaction of these cells to a regular surface microstructure of 1 micron pitch and 1 micron depth was tested. After two days on the microstructured samples, all the cells showed a strong alignment to the topography of the surface. Transmission electron microscopy revealed that the cells either bridged the grooves or conformed to the surface structures. The latter confirms earlier investigations with porous subcutaneous implants, where the inflammatory reaction and the formation of a fibrous tissue capsule was reduced due to enhanced tissue adhesion.
由下层基质的地形特性诱导的接触导向在形态发生中非常重要，并且也影响组织细胞与植入材料的相互作用。自1910年首次发现这一现象以来，已经积累了大量证据。已经提出了几个主要假说来解释观察到的细胞行为。技术进步使研究人员能够生产具有确定和可控表面微观几何形状的纯基质。基于这些样本，可以证明形成粘着斑的细胞骨架结构和受体最有可能参与接触导向。在一项使用人牙龈成纤维细胞的研究中，测试了这些细胞对1微米间距和1微米深度的规则表面微观结构的反应。在微结构化样本上培养两天后，所有细胞都表现出与表面形貌的强烈对齐。透射电子显微镜显示，细胞要么跨越凹槽，要么顺应表面结构。后者证实了早期对多孔皮下植入物的研究，在那里由于组织粘附增强，炎症反应和纤维组织囊的形成减少。
