Hintze D N, Farmer J L
J Bacteriol. 1975 Dec;124(3):1236-9. doi: 10.1128/jb.124.3.1236-1239.1975.
Bacillus subtilis strains 168 met ile leu and 23 thy contain folates which differ from one another in the number of glutamyl residues. The folate species were identified by reductive cleavage to the corresponding p-aminobenzoylglutamyl poly-gamma-glutamates and chromatography on diethylaminoethyl-cellulose. Pteroyltriglutamate is the predominant folate type, accounting for 86 to 88% of the total. Pteroyltetraglutamate is the only other type present in appreciable quantities, accounting for 5 to 6% of the total folates. Pteroyldiglutamate and pteroylpentaglutamate are present in small amounts, accounting for 1 to 3% and 1% of the total folates, respectively. Strain 168 met ile leu contains a very small amount of pteroylmonoglutamate (less than 0.5% of the total folates), but the other strain contains none.
枯草芽孢杆菌菌株168 met ile leu和23 thy所含的叶酸在谷氨酰残基数量上彼此不同。通过还原裂解为相应的对氨基苯甲酰谷氨酰多聚γ-谷氨酸，并在二乙氨基乙基纤维素上进行色谱分析来鉴定叶酸种类。蝶酰三谷氨酸是主要的叶酸类型，占总量的86%至88%。蝶酰四谷氨酸是唯一大量存在的其他类型，占总叶酸的5%至6%。蝶酰二谷氨酸和蝶酰五谷氨酸含量较少，分别占总叶酸的1%至3%和1%。菌株168 met ile leu含有极少量的蝶酰单谷氨酸（占总叶酸的不到0.5%），但另一个菌株不含。
