Juy D, Chedid L
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4105-9. doi: 10.1073/pnas.72.10.4105.
It has repeatedly been observed that various bacterial preparations could increase the host's resistance to tumors. It has also been shown that after nonspecific activation by BCG (bacillus Calmette-Guérin), peritoneal macrophages could inhibit in vitro the growth of neoplastic target cells. In the present study a fraction extracted from Myobacterium smegmatis and referred to as interphase material was tested in view of measuring its ability to activate macrophages in vitro and in vivo. This preparation was previously shown to protect mice against a syngeneic leukemia and to increase the immune response of the guinea pig. Other water-soluble adjuvants devoid of demonstrable antitumor activity in vivo were also assayed. The results argue in favor of a correlation between adjuvant activity and the capacity of activating macrophages. Moreover, interphase material administered in vivo consistently induced stronger and more persistent stimulations of macrophages than the other preparations assayed.
人们反复观察到,各种细菌制剂可增强宿主对肿瘤的抵抗力。研究还表明,经卡介苗(BCG)非特异性激活后,腹腔巨噬细胞可在体外抑制肿瘤靶细胞的生长。在本研究中,为了测定从耻垢分枝杆菌中提取的一种称为中间相物质的组分在体外和体内激活巨噬细胞的能力,对其进行了测试。该制剂先前已被证明可保护小鼠免受同基因白血病的侵害,并增强豚鼠的免疫反应。还对其他在体内无明显抗肿瘤活性的水溶性佐剂进行了检测。结果表明佐剂活性与激活巨噬细胞的能力之间存在相关性。此外,与其他检测制剂相比,体内给予中间相物质能持续诱导更强且更持久的巨噬细胞刺激。