Germano P, Pezzini A, Boccagni P, Zanoni G, Tridente G
Institute of Immunology and Infectious Diseases, University of Verona, Italy.
Int J Clin Lab Res. 1993;23(4):206-11. doi: 10.1007/BF02592310.
Serum antibodies to four common food antigens, three cows' milk proteins (casein, alpha-lactalbumin and beta-lactoglobulin) and ovalbumin, were investigated in 21 children with atopic dermatitis (aged 3 months to 3 years) and in 15 age-matched healthy controls. Specific IgE was measured by radioallergosorbent test; an ELISA was developed to detect specific IgG, IgG subclasses and IgA. Specific IgE was found in 76% of patients, while antigen-directed IgG and IgA were present both in patients and healthy controls; IgG to ovalbumin and IgA to alpha-lactalbumin were significantly higher in children with atopic dermatitis. The analysis of the IgG subclass distribution showed different patterns of response, IgG1 and IgG4 being higher in patients (even though statistically significant only for ovalbumin), and IgG2 and IgG3 being lower in this group. The presence of food-specific IgE in the majority of atopic children and the different specific IgG subclass patterns observed in patients and controls may reflect an alteration in the immune response to dietary proteins in atopic dermatitis.
对21名患有特应性皮炎的儿童（年龄在3个月至3岁之间）和15名年龄匹配的健康对照者，研究了针对四种常见食物抗原、三种牛奶蛋白（酪蛋白、α-乳白蛋白和β-乳球蛋白）和卵清蛋白的血清抗体。通过放射变应原吸附试验测定特异性IgE；开发了一种酶联免疫吸附测定法来检测特异性IgG、IgG亚类和IgA。76%的患者检测到特异性IgE，而抗原特异性IgG和IgA在患者和健康对照者中均有存在；患有特应性皮炎的儿童中，针对卵清蛋白的IgG和针对α-乳白蛋白的IgA显著更高。IgG亚类分布分析显示出不同的反应模式，患者中IgG1和IgG4更高（尽管仅对卵清蛋白具有统计学意义），而该组中IgG2和IgG3更低。大多数特应性儿童中存在食物特异性IgE，以及在患者和对照者中观察到的不同特异性IgG亚类模式，可能反映了特应性皮炎患者对膳食蛋白免疫反应的改变。
