Procunier J D, Tartof K D
Genetics. 1975 Nov;81(3):515-23. doi: 10.1093/genetics/81.3.515.
The 5S RNA genes of Drosophila melanogaster in either an isogenic wildtype or a multiply inverted (SM1) chromosome 2 increase their multiplicity when opposite a deficiency for the 5S gene site. This is analogous to the compensation phenomenon previously described for the 18S and 28S ribosomal RNA genes of the X chromosome nucleolus organizer region. Molecular hybridization of 5S RNA to DNA containing various doses of the 56F1-9 region of chromosome 2 demonstrates that most, if not all, of the 5S genes reside in or near this region. Also, a deficiency missing approximately one-half of the wild-type number of 5S genes was isolated and genetically localized. This mutant has a phenotype like that of bobbed, a mutant known to be partially deficient in 18S and 28S ribosomal RAN genes. Finally, we report the existence of a chromosomal rearrangement which splits the second chromosome into two segments, each containing 5S DNA.
果蝇（Drosophila melanogaster）的5S RNA基因，无论是在同基因野生型中，还是在多重倒位（SM1）的第二染色体中，当与5S基因位点的缺失相对时，其拷贝数都会增加。这类似于先前描述的X染色体核仁组织区18S和28S核糖体RNA基因的补偿现象。用5S RNA与含有不同剂量第二染色体56F1-9区域的DNA进行分子杂交表明，大部分（如果不是全部的话）5S基因位于该区域内或附近。此外，还分离出了一个缺失约一半野生型5S基因数量的缺失突变体，并对其进行了遗传定位。这个突变体的表型类似于截毛突变体，已知截毛突变体在18S和28S核糖体RNA基因中存在部分缺失。最后，我们报告了一种染色体重排的存在，它将第二条染色体分成两个片段，每个片段都含有5S DNA。
