Nagahiro S, Goto S, Korematsu K, Sumi M, Takahashi M, Ushio Y
Laboratory for Cerebrovascular Disorders and Neuroscience, Kumamoto University Medical School, Japan.
Brain Res. 1994 Jan 7;633(1-2):305-11. doi: 10.1016/0006-8993(94)91553-9.
The influence of transient cerebral ischemia on blood-brain and blood-cerebrospinal fluid (CSF) barrier permeability was studied sequentially by magnetic resonance imaging (MRI) contrast enhancement using gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA) in rats. The unilateral internal carotid and middle cerebral arteries were transiently occluded by inserting a nylon thread into the carotid artery and removing it following a variable interval of 5 to 60 min. Contrast enhancement of the lateral ventricle on the affected side was seen in the enhanced T1-weighted image at the early stage of reperfusion 6 h after the start of ischemia in most of the rats subjected to 30- and 60-min ischemia, and in 3 of 6 rats in the 15-min ischemia group. Autoradiograms of Gd-[14C]DTPA in rats subjected to 60-min ischemia demonstrated that the tracer strongly accumulated in the choroid plexus, the wall of the lateral ventricle and its surrounding brain tissue. On the other hand, parenchymal enhancement of the striatum was seen only in the 60-min ischemia group and appeared later on Day 1 or Day 7. These results indicate that ventricular enhancement on MRI in this model is caused by disruption of the blood-CSF barrier at the choroid plexus of the lateral ventricle. This is the first reported study to demonstrate blood-CSF barrier disruption by transient ischemia.
采用钆喷酸葡胺（Gd-DTPA），通过磁共振成像（MRI）对比增强技术，在大鼠中连续研究了短暂性脑缺血对血脑屏障和血脑脊液（CSF）屏障通透性的影响。通过将尼龙线插入颈动脉并在5至60分钟的可变间隔后取出，短暂阻断单侧颈内动脉和大脑中动脉。在大多数经历30分钟和60分钟缺血的大鼠以及15分钟缺血组的6只大鼠中的3只中，在缺血开始后6小时再灌注早期的增强T1加权图像中可见患侧侧脑室的对比增强。对经历60分钟缺血的大鼠进行的Gd-[14C]DTPA放射自显影显示，示踪剂强烈积聚在脉络丛、侧脑室壁及其周围脑组织中。另一方面，仅在60分钟缺血组中可见纹状体实质增强，且在第1天或第7天出现较晚。这些结果表明，该模型中MRI上的脑室增强是由侧脑室脉络丛处的血脑脊液屏障破坏引起的。这是首次报道的证明短暂性缺血导致血脑脊液屏障破坏的研究。
