Gamaleya N B
State Research Center of Addiction, Moscow, Russia.
Forensic Sci Int. 1993 Dec;63(1-3):285-93. doi: 10.1016/0379-0738(93)90282-f.
An ELISA method for detection of specific antibodies to morphine-protein conjugate (MPC) in humans and animals was elaborated. In humans, antibodies could be detected predominantly as the IgM class of immunoglobulins. Elevated levels of such antibodies were found in about 50% of opiate addicts and morphine-treated rats. In the case of prolonged and regular drug use with the time since the last drug intake not exceeding 2 months, elevated levels of antibodies to MPC were detected more frequently (up to 75%). Antibodies to MPC, detected in opiate addicts, were specific to MPC, morphine and beta-endorphin. A similar approach was applied to the detection of antibodies to ephedrone (amphetamine-like derivative of ephedrine) in case of its chronic use.
建立了一种用于检测人和动物体内吗啡 - 蛋白质结合物（MPC）特异性抗体的酶联免疫吸附测定（ELISA）方法。在人类中，抗体主要可检测为免疫球蛋白的IgM类。在约50%的阿片类药物成瘾者和经吗啡处理的大鼠中发现此类抗体水平升高。在长期规律用药且距上次用药时间不超过2个月的情况下，MPC抗体水平升高的检测更为频繁（高达75%）。在阿片类药物成瘾者中检测到的MPC抗体对MPC、吗啡和β - 内啡肽具有特异性。类似的方法被应用于检测麻黄酮（麻黄碱的苯丙胺样衍生物）长期使用情况下的抗体。
