Punnonen K, Ahotupa M, Asaishi K, Hyöty M, Kudo R, Punnonen R
Department of Clinical Chemistry, Central Laboratory University Central Hospital of Turku, Finland.
J Cancer Res Clin Oncol. 1994;120(6):374-7. doi: 10.1007/BF01247464.
We have analysed products of lipid peroxidation reactions and activities of antioxidant enzymes in cancerous breast tissue and in corresponding reference tissue. In addition, the serum lipid peroxidation and peroxyl-radical-trapping capacity of breast cancer patients were compared to those of healthy subjects. A total of 23 patients with breast cancer participated in this study. In the cancerous tissue, catalase activity was lower than in the reference tissue, while the activities of superoxide dismutase, glutathione peroxidase and the hexose monophosphate shunt were elevated. The content of thiobarbituric-acid-reactive material was slightly lower in the cancerous tissues, but the levels in serum were found to be elevated in patients with breast cancer. The amounts of conjugated diene double bonds were essentially equal both in the cancerous and in the reference tissue. Moreover, in breast cancer patients the serum levels of diene conjugation and the peroxyl-radical-scavenging capacity did not differ from those measured in healthy subjects. This study indicates that the antioxidant defence system is altered in cancerous breast tissues, but does not support the hypothesis suggesting that formation of lipid peroxides in the tumour tissue itself is of primary importance in the carcinogenesis.
我们分析了乳腺癌组织及相应对照组织中脂质过氧化反应产物和抗氧化酶的活性。此外，还比较了乳腺癌患者与健康受试者的血清脂质过氧化水平和过氧自由基捕获能力。共有23例乳腺癌患者参与了本研究。在癌组织中，过氧化氢酶活性低于对照组织，而超氧化物歧化酶、谷胱甘肽过氧化物酶和磷酸己糖旁路的活性升高。癌组织中硫代巴比妥酸反应性物质的含量略低，但乳腺癌患者血清中的该物质水平升高。共轭二烯双键的含量在癌组织和对照组织中基本相等。此外，乳腺癌患者血清中的二烯共轭水平和过氧自由基清除能力与健康受试者的测量值无差异。本研究表明，乳腺癌组织中的抗氧化防御系统发生了改变，但并不支持肿瘤组织本身脂质过氧化物的形成在致癌过程中起主要作用这一假说。
