DeShazo R D, Boyce J R, Osterman J V, Stephenson E H
JAMA. 1976 Mar 29;235(13):1353-5.
Diagnosis of Rocky Mountain spotted fever was made as early as the fourth day of febrile illness in rhesus monkeys (Macaca mulatta), with a monocyte culture technique that is easily amenable to use in humans. Rickettsia rickettsii were identified by direct immunofluorescent and Gimenez staining of cultures of circulating monocytes from four experimentally infected monkeys, even in the presence of a mild or inapparent disease in three. Levels of rickettsemia were estimated by the plaque-assay technique, and specific antibody titers were determined by complement fixation.
早在发热性疾病的第四天，就利用一种易于应用于人类的单核细胞培养技术，对恒河猴（猕猴）进行了落基山斑疹热的诊断。通过对四只实验感染猴子循环单核细胞培养物进行直接免疫荧光和吉姆尼兹染色，鉴定出了立氏立克次体，即使其中三只猴子患有轻度或不明显疾病。通过噬斑测定技术估计立克次体血症水平，并通过补体结合试验测定特异性抗体滴度。
