Patarca R, Klimas N G, Lugtendorf S, Antoni M, Fletcher M A
E. M. Papper Laboratory of Clinical Immunology, University of Miami School of Medicine, Florida.
Clin Infect Dis. 1994 Jan;18 Suppl 1:S147-53. doi: 10.1093/clinids/18.supplement_1.s147.
Among a group of 70 individuals who met the criteria established by the Centers for Disease Control and Prevention (Atlanta) for chronic fatigue syndrome (CFS), 12%-28% had serum levels exceeding 95% of control values for tumor necrosis factor (TNF) alpha, TNF-beta, interleukin (IL) 1 alpha, IL-2, soluble IL-2 receptor (sIL-2R), or neopterin; overall, 60% of patients had elevated levels of one or more of the nine soluble immune mediators tested. Nevertheless, only the distributions for circulating levels of TNF-alpha and TNF-beta differed significantly in the two populations. In patients with CFS--but not in controls--serum levels of TNF-alpha, IL-1 alpha, IL-4, and sIL-2R correlated significantly with one another and (in the 10 cases analyzed) with relative amounts (as compared to beta-globin or beta-actin) of the only mRNAs detectable by reverse transcriptase-coupled polymerase chain reaction in peripheral-blood mononuclear cells: TNF-beta, unspliced and spliced; IL-1 beta, lymphocyte fraction; and IL-6 (in order of appearance). These findings point to polycellular activation and may be relevant to the etiology and nosology of CFS.
在一组70名符合美国疾病控制与预防中心（亚特兰大）制定的慢性疲劳综合征（CFS）标准的个体中，12%至28%的人血清中肿瘤坏死因子（TNF）α、TNF-β、白细胞介素（IL）1α、IL-2、可溶性IL-2受体（sIL-2R）或新蝶呤的水平超过对照值的95%；总体而言，60%的患者所检测的九种可溶性免疫介质中的一种或多种水平升高。然而，只有TNF-α和TNF-β的循环水平在这两个人群中的分布有显著差异。在CFS患者中（而非对照组），TNF-α、IL-1α、IL-4和sIL-2R的血清水平彼此之间显著相关，并且（在分析的10例病例中）与外周血单核细胞中通过逆转录酶偶联聚合酶链反应可检测到的唯一mRNA的相对量（与β-球蛋白或β-肌动蛋白相比）显著相关：TNF-β，未剪接和剪接的；IL-1β，淋巴细胞部分；以及IL-6（按出现顺序）。这些发现表明存在多细胞激活，可能与CFS的病因学和疾病分类学相关。
