Yamaguchi-Shinozaki K, Shinozaki K
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, Ibaraki, Japan.
Plant Cell. 1994 Feb;6(2):251-64. doi: 10.1105/tpc.6.2.251.
Two genes, rd29A and rd29B, which are closely located on the Arabidopsis genome, are differentially induced under conditions of dehydration, low temperature, high salt, or treatment with exogenous abscisic acid (ABA). It appears that rd29A has at least two cis-acting elements, one involved in the ABA-associated response to dehydration and the other induced by changes in osmotic potential, and that rd29B contains at least one cis-acting element that is involved in ABA-responsive, slow induction. We analyzed the rd29A promoter in both transgenic Arabidopsis and tobacco and identified a novel cis-acting, dehydration-responsive element (DRE) containing 9 bp, TACCGACAT, that is involved in the first rapid response of rd29A to conditions of dehydration or high salt. DRE is also involved in the induction by low temperature but does not function in the ABA-responsive, slow expression of rd29A. Nuclear proteins that specifically bind to DRE were detected in Arabidopsis plants under either high-salt or normal conditions. Different cis-acting elements seem to function in the two-step induction of rd29A and in the slow induction of rd29B under conditions of dehydration, high salt, or low temperature.
两个基因，rd29A和rd29B，紧密位于拟南芥基因组上，在脱水、低温、高盐或用外源脱落酸（ABA）处理的条件下被差异诱导。似乎rd29A至少有两个顺式作用元件，一个参与与ABA相关的脱水反应，另一个由渗透势变化诱导，并且rd29B包含至少一个参与ABA响应性慢诱导的顺式作用元件。我们在转基因拟南芥和烟草中分析了rd29A启动子，并鉴定出一个包含9个碱基对TACCGACAT的新型顺式作用脱水响应元件（DRE），它参与rd29A对脱水或高盐条件的首次快速反应。DRE也参与低温诱导，但在rd29A的ABA响应性慢表达中不起作用。在高盐或正常条件下的拟南芥植物中检测到了与DRE特异性结合的核蛋白。不同的顺式作用元件似乎在rd29A的两步诱导以及脱水、高盐或低温条件下rd29B的慢诱导中发挥作用。
