从血清中分离丙型肝炎病毒RNA用于逆转录-聚合酶链反应。
Isolation of hepatitis C virus RNA from serum for reverse transcription-PCR.
作者信息
Nolte F S, Thurmond C, Mitchell P S
机构信息
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322.
出版信息
J Clin Microbiol. 1994 Feb;32(2):519-20. doi: 10.1128/jcm.32.2.519-520.1994.
Standard multistep extraction and isolation of RNA for hepatitis C virus (HCV) reverse transcription (RT)-PCR are impractical for routine use in clinical laboratories. We compared three simple commercially available methods for RNA isolation (RNAzol B, TRISOLV, and ULTRASPEC; Biotecx Laboratories, Houston, Tex.) and a total nucleic acid isolation method (IsoQuick; MicroProbe Corp., Garden Grove, Calif.) for the recovery of HCV RNA from sera obtained from 12 viremic patients for RT-PCR. RNAzol B, TRISOLV, ULTRASPEC, and IsoQuick extraction methods detected 87.5, 79.2, 33.3, and 58.3% of the paired positive samples, respectively. The method used for isolation of RNA is an important concern when optimizing HCV RT-PCR.
用于丙型肝炎病毒（HCV）逆转录（RT）-PCR的标准多步RNA提取和分离方法对于临床实验室的常规使用来说并不实用。我们比较了三种简单的市售RNA分离方法（RNAzol B、TRISOLV和ULTRASPEC；Biotecx Laboratories，休斯顿，德克萨斯州）和一种总核酸分离方法（IsoQuick；MicroProbe Corp.，加登格罗夫，加利福尼亚州），以从12名病毒血症患者的血清中提取HCV RNA用于RT-PCR。RNAzol B、TRISOLV、ULTRASPEC和IsoQuick提取方法分别检测出配对阳性样本的87.5%、79.2%、33.3%和58.3%。在优化HCV RT-PCR时，用于RNA分离的方法是一个重要的考虑因素。
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