Siroták L, Inoue K, Okada M, Amano T
Immunology. 1976 Mar;30(3):435-41.
Guinea-pigs produced both gamma1 and gamma2 antibodies against Escherichia coli K12, W3110/SM when they were injected intraperitoneally with a suspension of heat-killed bacteria without adjuvant twice a week for 12 weeks. The antibodies were separated by DEAE-cellulose chromatography. The gamma1 antibodies gave PCA reaction against soluble antigens prepared from sonicated E. coli, but the gamma2 antibodies did not. Both gamma1 and gamma2 antibodies induced complement-mediated bactericidal reaction against the homologous bacteria. In the case of gamma1 antibodies presensitization of bacteria with the antibodies before the addition of complement enhanced their bactericidal activity to a degree, EGTA inhibited the bactericidal activity of gamma2 antibodies, more than that of gamma1 antibodies. It is suggested that the gamma1 antibodies kill bacteria via the alternative pathway of complement activation, whilst the gamma2 antibodies exert their bactericidal activity mainly through the classical pathway.
当豚鼠每周两次腹腔注射无佐剂的热灭活大肠杆菌K12、W3110/SM悬液，持续12周时，它们产生了针对这些细菌的γ1和γ2抗体。抗体通过DEAE-纤维素层析进行分离。γ1抗体对由超声破碎大肠杆菌制备的可溶性抗原有PCA反应，而γ2抗体则没有。γ1和γ2抗体均诱导针对同源细菌的补体介导的杀菌反应。就γ1抗体而言，在加入补体之前用抗体对细菌进行预致敏在一定程度上增强了它们的杀菌活性，EGTA对γ2抗体杀菌活性的抑制作用比对γ1抗体的抑制作用更强。提示γ1抗体通过补体激活的替代途径杀死细菌，而γ2抗体主要通过经典途径发挥其杀菌活性。
