Tonkiss J, Shultz P, Galler J R
Center for Behavioral Development & Mental Retardation, Boston University School of Medicine, MA 02118.
Physiol Behav. 1994 Feb;55(2):217-24. doi: 10.1016/0031-9384(94)90126-0.
Developing rats were either malnourished or adequately nourished during the prenatal period by feeding their dams diets of low (6% casein) or adequate (25% casein) protein content 5 weeks prior to mating and throughout pregnancy. All pups received adequate nutrition from the day of birth onwards. Male offspring were tested in one of two spatial navigation tests in the Morris water tank. In proximal-cue tests (postnatal days 16-20), the position of a platform, which provided a means to escape from swimming, was denoted by an obvious visual cue located directly on the platform. In distal-cue tests (postnatal days 20-27 and adult ages, days 70-71 and days 220-221), the escape platform was submerged below the surface of the water so that the rats were required to use extramaze visual cues to guide them to the platform. Neither proximal-cue nor distal-cue navigation was significantly impaired in the prenatally malnourished rats relative to controls, at any of the ages tested.
在交配前5周及整个孕期，通过给孕鼠喂食低蛋白含量（6%酪蛋白）或充足蛋白含量（25%酪蛋白）的日粮，使发育中的大鼠在产前处于营养不良或营养充足状态。所有幼崽从出生之日起均获得充足营养。雄性后代在莫里斯水迷宫的两项空间导航测试之一中接受测试。在近端线索测试（出生后第16 - 20天）中，提供逃离游泳途径的平台位置由直接位于平台上的明显视觉线索表示。在远端线索测试（出生后第20 - 27天以及成年期，第70 - 71天和第220 - 221天）中，逃生平台被淹没在水面以下，因此大鼠需要利用迷宫外部的视觉线索引导它们找到平台。在任何测试年龄，产前营养不良的大鼠相对于对照组，近端线索或远端线索导航均未受到显著损害。
