Gordon M T, Mee A P, Anderson D C, Sharpe P T
University of Manchester, Dept. of Cell and Structural Biology, Manchester, UK.
Bone Miner. 1992 Nov;19(2):159-74. doi: 10.1016/0169-6009(92)90923-2.
Paget's disease is a chronic disease of the skeleton which is believed to be caused by a persistent virus of the paramyxovirus family. There is still conflict as to the precise identity of the virus(es) involved in causing this disease. Our previous results using in situ hybridisation have implicated distemper as a possible cause of this disease. In order to confirm these results, we have reverse transcribed RNA from pagetic bone and have specifically amplified for distemper and measles sequences using the polymerase chain reaction (PCR) technique. We have found that 8/13 of the patients examined had distemper and 1/10 cases had measles nucleic acids sequestered within their bone cells. One individual was found to have both viruses sequestered in his bone cells. Dideoxy sequencing of the distemper virus PCR products revealed 2% base pair changes in the nucleic acid sequences relative to the Onderstpoort strain of canine distemper. We can conclude that in Paget's disease, canine distemper and possible other paramyxoviruses reside in bone cells and that the persistent nature of the virus may be due to mutations in the viral genome.
佩吉特氏病是一种骨骼的慢性疾病，据信是由副粘病毒科的一种持续性病毒引起的。关于引发这种疾病的病毒的确切身份仍存在争议。我们之前使用原位杂交的结果表明犬瘟热可能是这种疾病的一个病因。为了证实这些结果，我们从佩吉特氏病的骨组织中逆转录了RNA，并使用聚合酶链反应（PCR）技术特异性扩增了犬瘟热和麻疹序列。我们发现，在接受检查的13名患者中，有8名患者的骨细胞中存在犬瘟热核酸，10名患者中有1名的骨细胞中存在麻疹核酸。发现有一名个体的骨细胞中同时存在这两种病毒。对犬瘟热病毒PCR产物进行双脱氧测序显示，相对于犬瘟热 Onderstpoort 毒株，核酸序列中有2%的碱基对发生了变化。我们可以得出结论，在佩吉特氏病中，犬瘟热以及可能的其他副粘病毒存在于骨细胞中，并且病毒的持续性可能是由于病毒基因组中的突变。
