Zaitoun A M
Department of Pathology, Al Qassimi Hospital, Sharjah, UAE.
J Clin Pathol. 1993 Jul;46(7):684-5. doi: 10.1136/jcp.46.7.684.
Gastric biopsy specimens from 283 patients with ulcer and non-ulcer dyspepsia attending five gastroenterology clinics in the northern region of the United Arab Emirates (UAE) were tested by the agar gel test (n = 115) or the ultra-rapid endoscopy room test (n = 168) for the presence of Helicobacter pylori urease. Results were compared with a histological technique using the Romanowsky type (Diff-3) stain for detecting H pylori in both antral and body type gastric mucosa. A sensitivity of 94% and specificity of 100% using the agar gel test compared with 87% sensitivity and 99.3% specificity for the ultra-rapid endoscopy room test. Grading of H pylori in gastric biopsy specimens showed that the higher the histological grade, the more likely that the urease test would be positive. Both forms of urease tests have high specificity for detecting H pylori in gastric biopsy specimens, although the urea agar test has a higher sensitivity than the ultra-rapid test. Low numbers of H pylori in gastric biopsy specimens are the most important determinant of a false negative urease test.
对来自阿拉伯联合酋长国（阿联酋）北部地区五家胃肠病诊所的283例溃疡和非溃疡性消化不良患者的胃活检标本，采用琼脂凝胶试验（n = 115）或超快速内镜室试验（n = 168）检测幽门螺杆菌尿素酶的存在情况。将结果与使用罗曼诺夫斯基型（Diff-3）染色的组织学技术进行比较，以检测胃窦和胃体部胃黏膜中的幽门螺杆菌。琼脂凝胶试验的敏感性为94%，特异性为100%，而超快速内镜室试验的敏感性为87%，特异性为99.3%。胃活检标本中幽门螺杆菌的分级显示，组织学分级越高，尿素酶试验呈阳性的可能性就越大。两种形式的尿素酶试验对检测胃活检标本中的幽门螺杆菌都具有高特异性，尽管尿素琼脂试验的敏感性高于超快速试验。胃活检标本中幽门螺杆菌数量少是尿素酶试验假阴性的最重要决定因素。
