使用DNA中度重复序列对曲菌球患者的烟曲霉分离株进行分型。

Use of DNA moderately repetitive sequence to type Aspergillus fumigatus isolates from aspergilloma patients.

作者信息

Girardin H, Sarfati J, Kobayashi H, Bouchara J P, Latgé J P

机构信息

Unité de Mycologie, Institut Pasteur, Paris, France.

出版信息

J Infect Dis. 1994 Mar;169(3):683-5. doi: 10.1093/infdis/169.3.683.

DOI:10.1093/infdis/169.3.683

PMID:8158052

Abstract

Polymorphism of forty-seven sequential clinical isolates from 3 patients with an aspergilloma was analyzed. DNA from each isolate was digested with EcoRI and hybridized in Southern blots with a 32P-labeled nonribosomal DNA repetitive sequence. Most isolates from each patient displayed the same hybridization pattern. Southern blot patterns obtained with DNA repetitive sequences can be used to type clinical isolates of Aspergillus fumigatus and have shown that aspergilloma patients are most probably infected by a single strain.

摘要

对来自3例曲菌球患者的47株连续临床分离株的多态性进行了分析。用EcoRI消化每个分离株的DNA，并在Southern印迹中与32P标记的非核糖体DNA重复序列杂交。来自每位患者的大多数分离株显示出相同的杂交模式。用DNA重复序列获得的Southern印迹模式可用于对烟曲霉临床分离株进行分型，并且已表明曲菌球患者很可能被单一菌株感染。