Drut R M, Day S, Drut R, Meisner L
Department of Cytogenetics, State Laboratory of Hygiene, University of Wisconsin, Madison, Wisconsin 53706.
Pediatr Pathol. 1994 Jan-Feb;14(1):101-9. doi: 10.3109/15513819409022030.
Burkitt's lymphoma (BL) is the most frequent non-Hodgkin's lymphoma in children in Argentina. Although epidemiologic studies have linked Epstein-Barr virus (EBV) to more than 90% of African BL cases but to only 10-20% of American and French cases, increased EBV-specific antibody titers were demonstrated in 73% of Argentinian patients with BL. To characterize the relationship between EBV and BL in Argentina, we analyzed paraffin-embedded tissues from 16 cases of BL for the presence of EBV DNA using the polymerase chain reaction (PCR) and in situ hybridization (ISH). PCR analysis showed that only 4 of 16 specimens contained the EBV BamW fragment, and these specimens were all from cases diagnosed in 1984. Results of ISH performed with a specific biotinylated DNA probe against the NotI/PstI fragment of EBV correlated with the PCR findings. EBV sequences were detected with ISH in 70-90% of the tumor cells from the 4 positive cases. These data may suggest an epidemic outbreak of EBV-related BL in 1984 superimposed on sporadic cases of BL, for which EBV may not have been an essential factor. This study also demonstrates the value of using molecular techniques on archival tissue to track epidemiologic trends.
伯基特淋巴瘤（BL）是阿根廷儿童中最常见的非霍奇金淋巴瘤。尽管流行病学研究表明，超过90%的非洲BL病例与爱泼斯坦-巴尔病毒（EBV）有关，但美国和法国的病例中只有10%-20%与之相关，然而，73%的阿根廷BL患者显示EBV特异性抗体滴度升高。为了明确阿根廷EBV与BL之间的关系，我们采用聚合酶链反应（PCR）和原位杂交（ISH）技术，分析了16例BL石蜡包埋组织中EBV DNA的存在情况。PCR分析显示，16个标本中只有4个含有EBV BamW片段，这些标本均来自1984年诊断的病例。用针对EBV NotI/PstI片段的特异性生物素化DNA探针进行ISH的结果与PCR结果相关。在4例阳性病例的70%-90%的肿瘤细胞中，ISH检测到了EBV序列。这些数据可能表明，1984年发生了EBV相关BL的流行暴发，叠加在散发的BL病例之上，而EBV可能不是散发病例的必要因素。本研究还证明了对存档组织使用分子技术来追踪流行病学趋势的价值。
