Ellerbroek P M, Oudkerk Pool M, Ridwan B U, Dolman K M, von Blomberg B M, von dem Borne A E, Meuwissen S G, Goldschmeding R
Department of Immunological Haematology, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
J Clin Pathol. 1994 Mar;47(3):257-62. doi: 10.1136/jcp.47.3.257.
To study ulcerative colitis associated neutrophil cytoplasmic antibodies (p-ANCA) in respect of class and subclass distribution, antigen specificity, and (sub)cellular localisation of the antigen(s) to which these antibodies are directed.
p-ANCA positivity was determined using the standard indirect immunofluorescence test (IIFT). The immunoglobulin (Ig) subclass distribution of p-ANCA was investigated using monoclonal antibodies directed against IgG1, IgG2, IgG3, and IgG4. Intracellular antigen localisation studies were performed on (fractionated) neutrophils using antigen-specific antibodies.
In contrast to vasculitis associated ANCA, ulcerative colitis p-ANCA are mainly of IgG1 and IgG3 subclass and lack IgG4. Ulcerative colitis p-ANCA are myeloid specific. IIFT data indicate that the related antigen(s) seem(s) to be located not in the cytosol, but in the granules (most likely the azurophil granules) of the neutrophil.
p-ANCA in ulcerative colitis have a different immunoglobulin subclass distribution than the ANCA of systemic necrotising vasculitis and necrotising and crescentic glomerulonephritis. This may point to differences in immune regulation between these diseases. Both cathepsin G and lactoferrin are recognised by a subpopulation of ulcerative colitis p-ANCA. In our series, eight out of 36 (22%) of ulcerative colitis associated p-ANCA react with lactoferrin and seven (19.5%) other sera with cathepsin G. None of them recognised both antigens. The main target antigen(s) of ulcerative colitis p-ANCA still remain(s) to be identified.
研究溃疡性结肠炎相关中性粒细胞胞浆抗体（p-ANCA）的类别和亚类分布、抗原特异性以及这些抗体所针对抗原的（亚）细胞定位。
采用标准间接免疫荧光试验（IIFT）测定p-ANCA阳性。使用针对IgG1、IgG2、IgG3和IgG4的单克隆抗体研究p-ANCA的免疫球蛋白（Ig）亚类分布。使用抗原特异性抗体对（分级分离的）中性粒细胞进行细胞内抗原定位研究。
与血管炎相关的ANCA不同，溃疡性结肠炎p-ANCA主要为IgG1和IgG3亚类，缺乏IgG4。溃疡性结肠炎p-ANCA具有髓系特异性。IIFT数据表明相关抗原似乎不在中性粒细胞的胞浆中，而是位于其颗粒（很可能是嗜天青颗粒）中。
溃疡性结肠炎中的p-ANCA与系统性坏死性血管炎以及坏死性和新月形肾小球肾炎的ANCA具有不同的免疫球蛋白亚类分布。这可能表明这些疾病在免疫调节方面存在差异。组织蛋白酶G和乳铁蛋白均被溃疡性结肠炎p-ANCA的一个亚群识别。在我们的研究系列中，36例溃疡性结肠炎相关p-ANCA中有8例（22%）与乳铁蛋白反应，7例（19.5%）其他血清与组织蛋白酶G反应。它们均未识别两种抗原。溃疡性结肠炎p-ANCA的主要靶抗原仍有待确定。
