Kiviranta I, Tammi M, Jurvelin J, Arokoski J, Säämänen A M, Helminen H J
Department of Surgery, Kuopio University Hospital, Finland.
J Orthop Res. 1994 Mar;12(2):161-7. doi: 10.1002/jor.1100120203.
The recovery of articular cartilage from atrophy induced by joint immobilization was investigated in immature dogs. In a previous study, we showed that 11 weeks of immobilization of the knee (stifle) joint of young dogs reduced the concentration of articular cartilage glycosaminoglycans (GAGs) by 13-47%. In the present study, right hindlimbs from six female beagles were immobilized for 11 weeks, as in the previous study, and then were remobilized for 15 weeks. Cartilage from the knee joint was compared with cartilage from nonimmobilized knees of eight age-matched control beagles. Histological samples taken from 11 different locations of the knee joint were stained with safranin O, and microspectrophotometry was used to demonstrate distribution of GAGs in the tissue. After remobilization, GAG concentration was restored in the patellofemoral region and tibial condyles. On the summits of the femoral condyles, and especially at the periphery of the femoral condyles, GAG concentration remained 8-26% less than the control values. On the summits, the thickness of the uncalcified cartilage was as much as 15% less than in the age-matched controls. Consequently, the changes induced by unloading were reversible to a great extent, but a full restoration of articular cartilage was not obtained at all sites of the knee joint within the 15 weeks of remobilization. Immobilization of the skeletally immature joint therefore may affect the development of articular cartilage in such a way that very slow recovery or permanent alterations are induced.
在未成熟犬中研究了关节固定引起的关节软骨萎缩的恢复情况。在先前的一项研究中，我们发现幼龄犬膝关节固定11周可使关节软骨糖胺聚糖（GAG）浓度降低13%-47%。在本研究中，如先前研究一样，对6只雌性比格犬的右后肢进行11周的固定，然后再活动15周。将膝关节软骨与8只年龄匹配的对照比格犬未固定膝关节的软骨进行比较。从膝关节11个不同部位采集的组织学样本用番红O染色，并用显微分光光度法显示组织中GAG的分布。再活动后，髌股区域和胫骨髁的GAG浓度恢复。在股骨髁的顶点，尤其是股骨髁的周边，GAG浓度仍比对照值低8%-26%。在顶点处，未钙化软骨的厚度比年龄匹配的对照犬少多达15%。因此，卸载引起的变化在很大程度上是可逆的，但在再活动的15周内，膝关节的所有部位并未完全恢复关节软骨。因此，骨骼未成熟关节的固定可能会以导致非常缓慢的恢复或永久性改变的方式影响关节软骨的发育。
