[与内质网结合的大鼠肝脏多核糖体：核糖核酸酶敏感的核糖体 - 膜连接的证据]
[Rat liver polysomes bound to endoplasmic reticulum: evidence for an RNase sensitive ribosome-membrane linkage].
作者信息
Dissous C, Krembel J
出版信息
C R Acad Hebd Seances Acad Sci D. 1976 Jan 19;282(3):313-6.
PMID:816558
Abstract
Repeated low speed centrifugations of a post-mitochondrial supernatant from rat liver through discontinuous sucrose gradients in presence of cytoplasmic RNase inhibitor yield a purified rough endoplasmic reticulum fraction. This fraction contains loosely-associated ribosomes (up to 20%) which can be released from membranes by a slight RNase treatment.
摘要
在细胞质核糖核酸酶抑制剂存在的情况下，将大鼠肝脏线粒体后上清液通过不连续蔗糖梯度进行反复低速离心，可得到纯化的粗面内质网组分。该组分含有松散结合的核糖体（高达20%），通过轻微的核糖核酸酶处理可从膜上释放出来。
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