Cawley J C, Smith J, Goldstone A H, Emmines J, Hamblin J, Hough L
Clin Exp Immunol. 1976 Jan;23(1):78-82.
Seventy-two cases of typical chronic lymphocytic leukaemia were screened by electron microscopy for the presence of intracytoplasmic immunoglobulin crystals. Immunoglobulin inclusions were found in four cases. Immunofluorescent studies showed that the inclusions contained IgA in two cases and IgM in the other two patients. Lambda light chain specificity was demonstrated in all four cases. The ultrastructure of the inclusions was identical in each patient except that in one of the IgA cases the inclusions were found in the perinuclear cistern in addition to the more usual location within cisternae of rough endoplasmic reticulum. Surface immunofluorescence showed mu heavy chains in the two cases displaying IgM crystal formation, but in the two IgA patients, no alpha heavy chains were demonstrable at the cell surface. The possible significance of these findings is discussed in relation to the existing literature.
对72例典型慢性淋巴细胞白血病患者进行电子显微镜检查,以筛查胞质内免疫球蛋白晶体的存在情况。在4例患者中发现了免疫球蛋白包涵体。免疫荧光研究表明,其中2例患者的包涵体含有IgA,另外2例含有IgM。所有4例均显示为λ轻链特异性。除1例IgA患者外,其余患者包涵体的超微结构均相同,该例患者除在粗面内质网池内常见位置外,还在核周池中发现了包涵体。表面免疫荧光显示,2例出现IgM晶体形成的患者细胞表面有μ重链,但2例IgA患者细胞表面未检测到α重链。结合现有文献对这些发现的可能意义进行了讨论。