Shenin Iu D, Khimich G N
Antibiot Khimioter. 1993 Jun;38(6):19-24.
The published data on the use of HPLC in studies on the chemistry of polyene macrolide antibiotics are summarized and the conditions for HPLC, the use of various sorbents, the impact of the ratio of the organic solvents and buffer solutions, etc. are discussed comparatively. Several aspects of the use of HPLC are indicated i.e. the identification of polyene antibiotics (heptaenes, pentaenes and hexaenes), the standardization and investigation of the antibiotic homogeneity and quality, the purification of the antibiotics and the determination of the amphotericin B content in tissues and plasma.
总结了已发表的关于高效液相色谱法（HPLC）在多烯大环内酯类抗生素化学研究中的应用数据，并比较讨论了HPLC的条件、各种吸附剂的使用、有机溶剂与缓冲溶液比例的影响等。指出了HPLC使用的几个方面，即多烯抗生素（七烯、五烯和六烯）的鉴定、抗生素均匀性和质量的标准化及研究、抗生素的纯化以及组织和血浆中两性霉素B含量的测定。
