Sobhani I, Denizot Y, Hochlaf S, Rigaud D, Vatier J, Benveniste J, Lewin M J, Mignon M
Service de Gastroentérologie, Hôpital Bichat, Paris, France.
Gut. 1993 Aug;34(8):1051-6. doi: 10.1136/gut.34.8.1051.
The release of platelet activating factor (PAF-ACETHER or PAF) and its precursors in the gastric lumen was assessed in 13 normal subjects in basal condition and after stimulation by gastrin. Acid, pepsin, and sialic acid outputs were determined under the same conditions. Gastric juice was collected using a nasogastric tube after overnight fast in basal condition for 60 minutes, then under pentagastrin infusion (6 micrograms/kg/hr for 60 minutes). Platelet activating factor was detected at low concentration in 4/13 subjects under basal condition (mean (SEM) 1.2 (0.6) pg/hr) while high concentrations of lyso platelet activating factor (6.1 (1.8) microgram/hr) and of alkyl-acyl-glycerophosphocholine (AAGPC) (11.5 (3) micrograms/hr) were found in 13 and 11 subjects, respectively. Platelet activating factor was not detected during pentagastrin infusion, while lyso platelet activating factor and alkyl-acyl-glycerophosphocholine were detected in 13 and in 12 subjects, respectively. Compared with the basal condition these platelet activating factor precursors increased significantly (p < 0.001) going up to fivefold baseline (31.8 (6.8) micrograms/hr and 53 (9.3) micrograms/hr respectively) in response to pentagastrin. There was a positive correlation between platelet activating factor precursors and acid or pepsin output but not between platelet activating factor precursors and sialic acid. As sialic acid may be considered an index of mucus glycoprotein degradation, it seems that gastrin stimulation of gastric epithelial cells results in a concomittant secretion of platelet activating factor precursors, acid, and pepsin irrespective of mucus glycoprotein degradation.
在13名正常受试者的基础状态下以及胃泌素刺激后，评估了胃腔内血小板活化因子（PAF - 乙酰醚或PAF）及其前体的释放情况。在相同条件下测定了酸、胃蛋白酶和唾液酸的分泌量。在基础状态下，过夜禁食后使用鼻胃管收集胃液60分钟，然后在五肽胃泌素输注（6微克/千克/小时，持续60分钟）期间收集。在基础状态下，4/13的受试者检测到低浓度的血小板活化因子（平均（标准误）1.2（0.6）皮克/小时），而在13名和11名受试者中分别发现了高浓度的溶血血小板活化因子（6.1（1.8）微克/小时）和烷基 - 酰基 - 甘油磷酸胆碱（AAGPC）（11.5（3）微克/小时）。在五肽胃泌素输注期间未检测到血小板活化因子，而在13名和12名受试者中分别检测到了溶血血小板活化因子和烷基 - 酰基 - 甘油磷酸胆碱。与基础状态相比，这些血小板活化因子前体显著增加（p < 0.001），对五肽胃泌素的反应上升至基线的五倍（分别为31.8（6.8）微克/小时和53（9.3）微克/小时）。血小板活化因子前体与酸或胃蛋白酶分泌量之间存在正相关，但与唾液酸之间不存在正相关。由于唾液酸可被视为黏液糖蛋白降解的指标，似乎胃泌素对胃上皮细胞的刺激会导致血小板活化因子前体、酸和胃蛋白酶的伴随分泌，而与黏液糖蛋白降解无关。
