Hicks E J, Hughes B J
Am J Clin Pathol. 1976 Apr;65(4):540-6. doi: 10.1093/ajcp/65.4.540.
The relative sensitivities of radioimmunoassay, crossoverelectrophoresis, and agar gel immunodiffusion for detecting HBAg in unconcentrated versus concentrated serum samples are compared and discussed. The sensitivities of the various immunologic methods were enhanced severalfold in concentrated serum samples. This is a fundamental consideration in designing a screening program for detecting HBAg and anti-HBAg antibody in hospital patients and donors. The authors propose that serum samples that have marginal reactions or low counts (RIA) be concentrated with Lyphogel or MiniconTM-125 concentrator and retested before test results are reported. Specificity of positive results was studied by neutralization tests with human serum containing antibodies to HBAg.
比较并讨论了放射免疫测定、交叉电泳和琼脂凝胶免疫扩散法在检测未浓缩与浓缩血清样本中乙肝表面抗原（HBAg）时的相对敏感性。在浓缩血清样本中，各种免疫方法的敏感性提高了数倍。这是为医院患者和献血者设计检测HBAg及抗-HBAg抗体筛查项目时的一个基本考虑因素。作者建议，对于反应微弱或计数较低（放射免疫测定）的血清样本，先用Lyphogel或MiniconTM - 125浓缩器进行浓缩，然后在报告检测结果之前重新检测。通过用含抗HBAg抗体的人血清进行中和试验研究了阳性结果的特异性。
